CHARACTERIZATION OF HUMAN SERUM N-ACETYLMURAMYL-L-ALANINE AMIDASE PURIFIED BY AFFINITY-CHROMATOGRAPHY

被引:21
作者
DEPAUW, P [1 ]
NEYT, C [1 ]
VANDERWINKEL, E [1 ]
WATTIEZ, R [1 ]
FALMAGNE, P [1 ]
机构
[1] UNIV MONS,SERV CHIM BIOL,B-7000 MONS,BELGIUM
关键词
D O I
10.1006/prep.1995.1049
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Human serum N-acetylmuramyl-L-alanine amidase was purified to homogeneity by a relatively short procedure including affinity chromatography. For this purpose, a specific adsorbent was prepared by coupling the main substrate of the enzyme, N-acetyl-muramyl-L-alanine-gamma-D-glutamyl-L-meso-2, diaminopimelic acid, to a divinylsulfone agarose gel. The enzyme is unable to hydrolyze this muramylpeptide when it is attached as a ligand to the gel, whereas a high affinity is conserved. In addition to affinity chromatography, the presented purification scheme includes ion exchange chromatography on DEAE-Sepharose and molecular sieving on Superdex 200. The enzyme was purified 739-fold with a yield of 22.5%. One single band at 135 kDa was obtained on native gradient PAGE. Gradient PAGE in denaturing conditions gave one single band at 74 kDa, which was lowered to 64 kDa when the enzyme was denatured in nonreducing conditions, This suggests that the native enzyme is a dimer consisting of two subunits of identical molecular weight, with only intramolecular disulfide bonds. Isoelectric focusing gave one single band at pI 5.0. Glycan detection before and after treatment with N-glycosidase F showed that the enzyme is a glycoprotein. Further analysis by lectin immune detection on dot blots confirmed that the enzyme is an N-glycosylated protein of complex type with sialic acid, terminally linked alpha(2 --> 6) to galactose or N-acetylgalactosamine. The 15 amino acid N-terminal sequence was determined by microsequence analysis. (C) 1995 Academic Press, Inc.
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页码:371 / 378
页数:8
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共 42 条
[1]  
Ladesic B., Tomasic J., Kvcder S., Hrsak I., Metabolic fate of carbon-14 labeled immunoadjuvant peptidoglycan oligomer 2, Vitro Studies. Biochim. Biophys. Acta, 678, pp. 12-17, (1981)
[2]  
Mollner S., Braun V., Murein hydrolase (N-acetyl-muramyl-L-alanine amidase) in human serum, Arch. Microbiol, 140, pp. 171-177, (1984)
[3]  
Harisson J., Fox A., Degradation of muramyl dipeptide by mammalian serum, Infect. Immun, 50, pp. 320-321, (1985)
[4]  
Vanderwinkel E., De Vlieghere M., De Pauw P., Cattalini N., Ledoux V., Gigot D., Ten Have J.-P., Purification and characterization of N-acetylmuramoyl-L-alanine amidase from human serum, Biochim. Biophys. Acta, 1039, pp. 331-338, (1990)
[5]  
Johannsen L., Krueger J.M., Quantitation of dia-mino-pimelic acid in human urine, Adv. Biosci, 68, pp. 445-449, (1988)
[6]  
Krueger J.M., Pappenheimer J.R., Karnovsky M.L., The composition of sleep-promoting factor isolated from human urine, J. Biol. Chem, 257, pp. 1664-1669, (1982)
[7]  
Zhai S., Karnovsky M.L., Qualitative detection of muramic acid in normal mammalian tissues, Infect. Immun, 43, pp. 937-941, (1984)
[8]  
Roten C.-A.H., Karamata D., Endogenous synthesis of peptidoglycan in eukaryotic cells
[9]  
a novel concept involving its essential role in cell division, tumor formation and the biological clock, Experienta, 48, pp. 921-931, (1992)
[10]  
Gordon S., Todd J., Cohn Z.A., In vitro synthesis and secretion of lysozyme by mononuclear phagocytes, J. Exp. Med, 139, pp. 1228-1248, (1974)