IDENTIFICATION OF AXIAL LIGANDS OF CYTOCHROME C(552) FROM NITROSOMONAS-EUROPAEA

被引:21
作者
ARCIERO, DM
PENG, QY
PETERSON, J
HOOPER, AB
机构
[1] UNIV MINNESOTA, DEPT GENET & CELL BIOL, ST PAUL, MN 55108 USA
[2] UNIV ALABAMA, DEPT CHEM, TUSCALOOSA, AL 35487 USA
来源
FEBS LETTERS | 1994年 / 342卷 / 02期
关键词
CYTOCHROME C(552); NITROSOMONAS EUROPAEA; AXIAL LIGAND; 695 NM BAND; EPR; HALS SIGNAL; MCD; NEAR-INFRARED;
D O I
10.1016/0014-5793(94)80504-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cytochrome c(552) from Nitrosomonas europaea was analyzed by visible, EPR and MCD spectroscopies. The visible and MCD data show that histidine and methionine are the axial ligands to the heme iron of the ferric protein. The EPR spectrum of the cytochrome shows an atypical highly axial low spin (HALS) type signal with g-values that make it difficult to identify the axial ligands. These results reinforce the value of near-infrared MCD spectroscopy for assigning ligands in ferric heme systems and point out the difficulties in using only EPR spectroscopy for the same purpose. The description of another c-cytochrome exhibiting a HALS-type EPR signal will eventually be helpful in explaining the physical basis for this unusual signal.
引用
收藏
页码:217 / 220
页数:4
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