EFFECTS OF NEURAMINIDASE TREATMENT ON THE BIOLOGICAL-ACTIVITIES OF FACTOR-VIII

被引：13

作者：

ROSENFELD, L

KIRBY, EP

机构：

[1] TEMPLE UNIV,DEPT BIOCHEM,PHILADELPHIA,PA 19140

[2] TEMPLE UNIV,SPECIALIZED CTR THROMBOSIS RES,PHILADELPHIA,PA 19140

来源：

THROMBOSIS RESEARCH | 1979年 / 15卷 / 1-2期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0049-3848(79)90071-9

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Complete removal of sialic acid from bovine Factor VIII by treatment with neuraminidase results in no loss of platelet agglutinating factor activity or procoagulant activity. There is no alteration in the immunological properties of the molecule, as measured by double immunodiffusion or the Laurell assay, although its electrophoretic mobility is slightly decreased. Removal of sialic acid from human Factor VIII, however, results in a 74 percent loss of ristocetin cofactor activity. © 1979.

引用

页码：255 / 261

页数：7

共 18 条

[1]

ATICHARTAKARN V, 1978, BLOOD, V51, P281

[2] BINDING OF SOLUBLE FORM OF FIBROBLAST SURFACE PROTEIN, FIBRONECTIN, TO COLLAGEN [J].