DETECTION OF BLUETONGUE VIRUS IN CULICOIDES-VARIIPENNIS (DIPTERA, CERATOPOGONIDAE) BY AN ANTIGEN CAPTURE ENZYME-LINKED-IMMUNOSORBENT-ASSAY

被引：37

作者：

MECHAM, JO

DEAN, VC

WIGINGTON, JG

NUNAMAKER, RA

机构：

[1] Arthropod-borne Animal Diseases Research Laboratory, USDA-ARS, Laramie, 82071., Wyoming

来源：

JOURNAL OF MEDICAL ENTOMOLOGY | 1990年 / 27卷 / 04期

关键词：

D O I：

10.1093/jmedent/27.4.602

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

An antigen capture enzyme-linked immunosorbent assay (ELISA) was developed to detect bluetongue virus (BTV) in the vector Culicoides variipennis (Coquillett). This ELISA used polyclonal and monoclonal antibodies in a biotin-avidin immunoperoxidase system to detect BTV in individual adult female flies that had taken an infectious blood meal. Detection of BTV with the ELISA correlated with detection of BTV from the same flies by plaque assay in cell culture. The ELISA was group-specific for the five U.S. serotypes of BTV and did not cross react with closely related epizootic hemorrhagic disease virus.

引用

页码：602 / 606

页数：5

共 27 条

[1]

BARBER TL, 1979, AM J VET RES, V40, P1654

[2] COMPARISON OF VARIOUS IMMUNOHISTOCHEMICAL METHODS - DEMONSTRATION OF EXTRACELLULAR ANTIGENS IN CRYOSTAT SECTIONS [J].