LENS MEMBRANES .7. MIP IS AN IMMUNOLOGICALLY SPECIFIC COMPONENT OF LENS FIBER MEMBRANES AND IS IDENTICAL WITH 26K-BAND PROTEIN

被引：56

作者：

BROEKHUYSE, RM ^{[1
]}

KUHLMANN, ED ^{[1
]}

WINKENS, HJ ^{[1
]}

机构：

[1] CATHOLIC UNIV NIJMEGEN, INST OPHTHALMOL, NIJMEGEN, NETHERLANDS

来源：

EXPERIMENTAL EYE RESEARCH | 1979年 / 29卷 / 03期

关键词：

epithelium; fiber; immunochemical specificity; immunofluorescence; lens; main intrinsic protein; membrane proteins; organ specificity;

D O I：

10.1016/0014-4835(79)90009-5

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Calf lens MIP [main intrinsic polypeptide] (solvent-extracted from the fiber membranes) and 26,000 band protein (isolated by SDS-PAGE [sodium dodecyl sulfate-polyacrylamide gel electrophoresis]) had very similar amino acid compositions and MW. MIP comprises 2 components immunlogically identrical with the 2 antigens detected in 26,000 band protein. MIP extracts contain 2 antigens in very small amounts which are common to urea-treated fiber and epithelial lens membranes. The 26,000 band protein contains 1 of these antigens. Immunodiffusion, immunofluorescence and SDS-PAGE show MIP is a specific component of the fiber membranes, which is not detected in epithelial membranes. MIP is a marker of differentiation. MIP determinants only occur in the lens demonstrating the organ specificity of the protein.

引用

页码：303 / 313

页数：11

共 16 条

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