PLATELET-ACTIVATING-FACTOR INDUCES EXPRESSION OF EARLY RESPONSE GENES C-FOS AND TIS-1 IN HUMAN EPIDERMOID CARCINOMA A-431 CELLS

被引：33

作者：

TRIPATHI, YB ^{[1
]}

KANDALA, JC ^{[1
]}

GUNTAKA, RV ^{[1
]}

LIM, RW ^{[1
]}

SHUKLA, SD ^{[1
]}

机构：

[1] UNIV MISSOURI,SCH MED,DEPT MOLEC MICROBIOL & IMMUNOL,COLUMBIA,MO 65212

来源：

LIFE SCIENCES | 1991年 / 49卷 / 23期

关键词：

D O I：

10.1016/0024-3205(91)90319-7

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The effect of platelet activating factor (PAF) on the induction of early response genes was investigated in A-431 cells (human epidermal carcinoma cells). PAF induced a transient expression of c-fos and TIS-1 mRNA in a time- and dose-dependent manner. As low as 10(-10) M PAF caused detectable expression of these genes with a maximum observed at 10(-7) M. In the presence of cycloheximide, increases in the gene expression were noticeable at 20 min and peaked between 30-60 min. A lack of induction with lyso-PAF, an inactive PAF metabolite, confirmed the specificity of PAF towards this expression. The cells pretreated with CV-6209, a PAF receptor antagonist, did not show any induction of these genes by PAF. It is concluded that PAF causes induction of the early response genes c-fos and TIS-1 in a structurally specific and receptor dependent manner. This finding offers a new role for PAF at the nuclear level and may have important implications in the long term effects of PAF in pathophysiological conditions.

引用

页码：1761 / 1767

页数：7

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