ELECTROCHROMATOGRAPHIC SOLID-PHASE EXTRACTION FOR DETERMINATION OF CIMETIDINE IN SERUM BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

A highly effective electrochromatographic solid-phase extraction and preconcentration method is reported for the determination of cimetidine in serum in the concentration range 0.233-11.4-mu-g/ml. Preconcentrated samples were determined by micellar electrokinetic capillary chromatography while ranitidine was used as an internal standard. Sample preparation included retention of the analyte on a C18 solid-phase cartridge, followed by elution assisted by an applied voltage of 150 V. From 0.5-ml serum samples, 20-50-mu-l aliquots were collected for electrophoretic analysis. Within the studied concentration range, the method was linear and provided adequate precision.