ANALYSIS OF THE EXPRESSION OF A GLUCOSE-REGULATED PROTEIN (GRP78) PROMOTER CAT FUSION GENE DURING EARLY XENOPUS-LAEVIS DEVELOPMENT

被引：10

作者：

WINNING, RS

BOLS, NC

WOODEN, SK

LEE, AS

HEIKKILA, JJ

机构：

[1] UNIV WATERLOO,DEPT BIOL,WATERLOO N2L 3G1,ONTARIO,CANADA

[2] UNIV SO CALIF,SCH MED,NORRIS CANC RES INST,LOS ANGELES,CA 90033

[3] UNIV SO CALIF,SCH MED,DEPT BIOCHEM,LOS ANGELES,CA 90033

来源：

DIFFERENTIATION | 1992年 / 49卷 / 01期

关键词：

D O I：

10.1111/j.1432-0436.1992.tb00763.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Developmental regulation of the expression of a glucose-regulated gene encoding a 78 kd protein, GRP78, has been characterized by microinjection of a rat GRP78/CAT chimeric gene into early Xenopus embryos. Tunicamycin-induced expression of the chimeric gene during Xenopus development was similar to the pattern of endogenous GRP78 protein synthesis, with expression first being detected at gastrula and increasing at least until the tailbud stage. Deletion analysis of the rat GRP78 promoter revealed that sequences between -154 and -130 were necessary for full tunicamycin-inducible and constitutive expression of the fusion gene. These results suggest that there is conservation of regulatory elements of the GRP78 promoter between rat and Xenopus.

引用

页码：1 / 6

页数：6

共 31 条

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