COOPERATIVE OLIGONUCLEOTIDE-DIRECTED TRIPLE-HELIX FORMATION AT ADJACENT DNA SITES

被引：34

作者：

COLOCCI, N ^{[1
]}

DISTEFANO, MD ^{[1
]}

DERVAN, PB ^{[1
]}

机构：

[1] CALTECH, ARNOLD & MABEL BECKMAN LAB CHEM SYNTHESIS, DIV CHEM & CHEM ENGN, PASADENA, CA 91125 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1993年 / 115卷 / 11期

关键词：

D O I：

10.1021/ja00064a008

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The changes in binding free energies due to cooperativity for oligodeoxyribonucleotides bound to adjacent sites by triple helix formation have been determined by quantitative affinity cleavage titrations. A 20-fold enhancement in equilibrium association constant is realized for an 11 mer pyrimidine oligonucleotide binding in the presence of a neighboring bound site at 24-degrees-C and pH 7.0 (25 mM Tris-OAc, 10 mM NaCl, 1 mM spermine). This corresponds to an increase in binding free energy of 1.8 +/- 0.2 kcal mol-1. This cooperativity is not observed when the two binding sites are separated by one base pair.

引用

页码：4468 / 4473

页数：6

共 54 条

[1] MULTIPARTITE GENETIC-CONTROL ELEMENTS - COMMUNICATION BY DNA LOOP
ADHYA, S
[J]. ANNUAL REVIEW OF GENETICS, 1989, 23 : 227 - 250
[2] [Anonymous], 1989, MOL CLONING
[3] STRUCTURES FOR POLYNUCLEOTIDE COMPLEXES POLY(DA).POLY(DT) AND POLY(DT).POLY(DA).POLY(DT)
ARNOTT, S
SELSING, E
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1974, 88 (02) : 509 - +
[4] THE INFLUENCE OF SINGLE BASE TRIPLET CHANGES ON THE STABILITY OF A PUR-BULLET-PUR-BULLET-PYR TRIPLE HELIX DETERMINED BY AFFINITY CLEAVING
BEAL, PA
DERVAN, PB
[J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (11) : 2773 - 2776
[5] 2ND STRUCTURAL MOTIF FOR RECOGNITION OF DNA BY OLIGONUCLEOTIDE-DIRECTED TRIPLE-HELIX FORMATION
BEAL, PA
DERVAN, PB
[J]. SCIENCE, 1991, 251 (4999) : 1360 - 1363
[6] PREDICTING DNA DUPLEX STABILITY FROM THE BASE SEQUENCE
BRESLAUER, KJ
FRANK, R
BLOCKER, H
MARKY, LA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (11) : 3746 - 3750
[7] INTERACTION AND ASSOCIATION OF BASES AND NUCLEOSIDES IN AQUEOUS SOLUTIONS .V. STUDIES OF ASSOCIATION OF PURINE NUCLEOSIDES BY VAPOR PRESSURE OSMOMETRY AND BY PROTON MAGNETIC RESONANCE
BROOM, AD
SCHWEIZE.MP
TSO, POP
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1967, 89 (14) : 3612 - &
[8] Cantor CR., 1980, BIOPHYSICAL CHEM 3
[9] SITE-SPECIFIC INTERCALATION AT THE TRIPLEX-DUPLEX JUNCTION INDUCES A CONFORMATIONAL CHANGE WHICH IS DETECTABLE BY HYPERSENSITIVITY TO DIETHYLPYROCARBONATE
COLLIER, DA
MERGNY, JL
THUONG, NT
HELENE, C
[J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (15) : 4219 - 4224
[10] SITE-SPECIFIC OLIGONUCLEOTIDE BINDING REPRESSES TRANSCRIPTION OF THE HUMAN C-MYC GENE INVITRO
COONEY, M
CZERNUSZEWICZ, G
POSTEL, EH
FLINT, SJ
HOGAN, ME
[J]. SCIENCE, 1988, 241 (4864) : 456 - 459

← 1 2 3 4 5 6 →