ORIGIN OF CAMP-DEPENDENT C1(-) SECRETION FROM BOTH CRYPTS AND SURFACE EPITHELIA OF RAT INTESTINE

被引:90
作者
KOCKERLING, A [1 ]
FROMM, M [1 ]
机构
[1] FREE UNIV BERLIN, KLINIKUM STEGLITZ, INST KLIN PHYSIOL, HINDENBURGDAMM 30, W-1000 BERLIN 45, GERMANY
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1993年 / 264卷 / 05期
关键词
SECRETORY DIARRHEA; VOLTAGE SCANNING; LOCAL ION CONDUCTANCE; SHORT-CIRCUIT CURRENT; CRYPT MORPHOMETRY; THEOPHYLLINE; ADENOSINE; 3'; 5'-CYCLIC MONOPHOSPHATE; PROSTAGLANDIN-E1; 5-NITRO-2-(3-PHENYLPROPYLAMINO)BENZOIC ACID; LATE DISTAL COLON; ILEUM;
D O I
10.1152/ajpcell.1993.264.5.C1294
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Adenosine 3',5'-cyclic monophosphate (cAMP)-dependent Cl- secretion provides the ionic basis for secretory diarrhea. We quantified the spatial distribution of this process by measuring local ion conductance in crypts and surface epithelium or villi of rat late distal colon and ileum. By use of an improved voltage-scanning technique, the tissue was clamped to a 30-Hz sine-wave current and the electrical field above the respective structures was sensed by a stepping glass microelectrode. Under control conditions, crypts and surface epithelium contributed 61 and 39%, respectively, to the total ion conductance of distal colon. Theophylline (10 mM) increased crypt conductance (Gc) by 64% from 2.5 +/- 0.2 to 4.1 +/- 0.3 mS/cm2 and surface epithelium conductance (G(s)) by 69% from 1.6 +/- 0.1 to 2.7 +/- 0.1 mS/cm2. These changes in local conductances were completely Cl- dependent, since theophylline had no effect when Cl- was replaced by gluconate. Similar results were obtained when Cl- secretion was elicited by prostaglandin E1 (1 muM) or by dibutyryl-cAMP (DBcAMP, 1 mM). After stimulation, the Cl- channel blocker 5-nitro-2-(3-phenyl-propylamino) benzoic acid (1 mM) decreased both G(c) and G(s). In rat ileum, theophylline plus DBcAMP caused an increase in total conductance of 19% only because of its large paracellular conductance. The ratio of scanning signals above villi and intervillous spaces was unaffected, indicating that Cl- conductance is induced in both crypts and villi. We conclude that in distal large intestine cAMP-dependent Cl- secretion is not confined to crypts but is evenly performed also by surface cells. A similar distribution exists in small intestine.
引用
收藏
页码:C1294 / C1301
页数:8
相关论文
共 33 条
[1]   SUBMUCOSAL PLEXUS AND ELECTROLYTE TRANSPORT ACROSS RAT COLONIC MUCOSA [J].
ANDRES, H ;
BOCK, R ;
BRIDGES, RJ ;
RUMMEL, W ;
SCHREINER, J .
JOURNAL OF PHYSIOLOGY-LONDON, 1985, 364 (JUL) :301-+
[2]   CAMP-MEDIATED EFFECTS OF OUABAIN AND THEOPHYLLINE ON PARACELLULAR ION SELECTIVITY [J].
BAKKER, R ;
GROOT, JA .
AMERICAN JOURNAL OF PHYSIOLOGY, 1984, 246 (02) :G213-G217
[3]   ALTERED INTESTINAL CHLORIDE TRANSPORT IN CYSTIC-FIBROSIS [J].
BERSCHNEIDER, HM ;
KNOWLES, MR ;
AZIZKHAN, RG ;
BOUCHER, RC ;
TOBEY, NA ;
ORLANDO, RC ;
POWELL, DW .
FASEB JOURNAL, 1988, 2 (10) :2625-2629
[4]   OCCLUDING JUNCTIONS IN A CULTURED TRANSPORTING EPITHELIUM - STRUCTURAL AND FUNCTIONAL-HETEROGENEITY [J].
CEREIJIDO, M ;
STEFANI, E ;
MARTINEZPALOMO, A .
JOURNAL OF MEMBRANE BIOLOGY, 1980, 53 (01) :19-32
[5]   SINGLE CHLORIDE CHANNELS IN COLON MUCOSA AND ISOLATED COLONIC ENTEROCYTES OF THE RAT [J].
DIENER, M ;
RUMMEL, W ;
MESTRES, P ;
LINDEMANN, B .
JOURNAL OF MEMBRANE BIOLOGY, 1989, 108 (01) :21-30
[6]  
DONOWITZ M, 1982, GASTROENTEROLOGY, V83, P1231
[7]  
DONOWITZ M, 1987, PHYSL GASTROINTESTIN, V2, P1351
[8]   INTESTINAL ION-TRANSPORT AND DIARRHEAL DISEASE [J].
FONDACARO, JD .
AMERICAN JOURNAL OF PHYSIOLOGY, 1986, 250 (01) :G1-G8
[9]  
FOSKETT JK, 1989, METHOD ENZYMOL, V171, P792
[10]   ROUTE OF PASSIVE ION MOVEMENT THROUGH EPITHELIUM OF NECTURUS GALLBLADDER [J].
FROMTER, E .
JOURNAL OF MEMBRANE BIOLOGY, 1972, 8 (03) :259-+