LIGHT-EMITTING-DIODES AS A LIGHT-SOURCE FOR PHOTOSYNTHESIS RESEARCH

Light-emitting diodes (LED) can provide large fluxes of red photons and so could be used to make lightweight, efficient lighting systems for photosynthetic research. We compared photosynthesis, stomatal conductance and isoprene emission (a sensitive indicator of ATP status) from leaves of kudzu (Pueraria lobata (Willd) Ohwi.) enclosed in a leaf chamber illuminated by LEDs versus by a xenon are lamp. Stomatal conductance was measured to determine if red LED light could sufficiently open stomata. The LEDs produced an even field of red light (peak emission 656 +/- 5 nm) over the range of 0-1500 mu mol m(-2) s(-1). Under ambient CO2 the photosynthetic response to red light deviated slightly from the response measured in white light and stomatal conductance followed a similar pattern. Isoprene emission also increased with light similar to photosynthesis in white light and red light. The response of photosynthesis to CO2 was similar under the LED and xenon are lamps at equal photosynthetic irradiance of 1000 mu mol m(-2) s(-1). There was no statistical difference between the white light and red light measurements in high CO2. Some leaves exhibited feedback inhibition of photosynthesis which was equally evident under irradiation of either lamp type. Photosynthesis research including electron transport, carbon metabolism and trace gas emission studies should benefit greatly from the increased reliability, repeatability and portability of a photosynthesis lamp based on light-emitting diodes.