GLUTAMATE-GATED CHLORIDE CHANNEL WITH GLUTAMATE-TRANSPORTER-LIKE PROPERTIES IN CONE PHOTORECEPTORS OF THE TIGER SALAMANDER

被引:91
作者
PICAUD, SA [1 ]
LARSSON, HP [1 ]
GRANT, GB [1 ]
LECAR, H [1 ]
WERBLIN, FS [1 ]
机构
[1] UNIV CALIF BERKELEY, DEPT MOLEC & CELL BIOL, DIV NEUROBIOL, BERKELEY, CA 94720 USA
关键词
D O I
10.1152/jn.1995.74.4.1760
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. Using the patch-clamp technique, we investigated whether the glutamate-elicited current in mechanically isolated cone photoreceptors from the salamander retina is generated by a Cl- channel or a glutamate transporter. 2. The current reversed near the equilibrium potential for Cl-, was decreased by three Cl- channel blockers, 5-nitro-2-(3-phenyl-propylamino) benzoic acid, 4,4'-diisothiocyanostilbene-2,2'-disulfonate, and diphenylamine 2,2'-dicarboxylic acid, and was eliminated when gluconate was substituted for both internal and external Cl-, features consistent with the current being mediated by a Cl- channel. 3. The single-channel conductance of the Cl- channel was estimated by noise analysis of the glutamate-elicited current fluctuations to be 0.7 pS with an open time of 2 ms. 4. The magnitude of the current was dependent on both internal and external Na+ and K+, features consistent with the current being related to the activation of a glutamate transporter. Yet changes in their concentrations did not affect the reversal potential of the current. 5. Taken together with earlier reports on this current showing a that it has a glutamate-transporter-like pharmacology, our results suggest that the glutamate-elicited current is carried by a Cl- channel but gated by a glutamate receptor whose pharmacology and ionic requirement resemble those previously described for glutamate transporters.
引用
收藏
页码:1760 / 1771
页数:12
相关论文
共 37 条
  • [1] ATP-DEPENDENT BACTERIAL TRANSPORTERS AND CYSTIC-FIBROSIS - ANALOGY BETWEEN CHANNELS AND TRANSPORTERS
    AMES, GF
    LECAR, H
    [J]. FASEB JOURNAL, 1992, 6 (09) : 2660 - 2666
  • [2] DEMONSTRATION THAT CFTR IS A CHLORIDE CHANNEL BY ALTERATION OF ITS ANION SELECTIVITY
    ANDERSON, MP
    GREGORY, RJ
    THOMPSON, S
    SOUZA, DW
    PAUL, S
    MULLIGAN, RC
    SMITH, AE
    WELSH, MJ
    [J]. SCIENCE, 1991, 253 (5016) : 202 - 205
  • [3] AYOUB GS, 1989, NEUROSCIENCE RES S, V10, P47
  • [4] ELECTROGENIC UPTAKE OF GLUTAMATE AND ASPARTATE INTO GLIAL-CELLS ISOLATED FROM THE SALAMANDER (AMBYSTOMA) RETINA
    BARBOUR, B
    BREW, H
    ATTWELL, D
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 1991, 436 : 169 - 193
  • [5] ELECTROGENIC GLUTAMATE UPTAKE IN GLIAL-CELLS IS ACTIVATED BY INTRACELLULAR POTASSIUM
    BARBOUR, B
    BREW, H
    ATTWELL, D
    [J]. NATURE, 1988, 335 (6189) : 433 - 435
  • [6] THE GLIAL-CELL GLUTAMATE UPTAKE CARRIER COUNTERTRANSPORTS PH-CHANGING ANIONS
    BOUVIER, M
    SZATKOWSKI, M
    AMATO, A
    ATTWELL, D
    [J]. NATURE, 1992, 360 (6403) : 471 - 474
  • [7] L-GLUTAMIC ACID - A NEUROTRANSMITTER CANDIDATE FOR CONE PHOTORECEPTORS IN HUMAN AND RAT RETINAS
    BRANDON, C
    LAM, DMK
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (16): : 5117 - 5121
  • [8] ELECTROGENIC GLUTAMATE UPTAKE IS A MAJOR CURRENT CARRIER IN THE MEMBRANE OF AXOLOTL RETINAL GLIAL-CELLS
    BREW, H
    ATTWELL, D
    [J]. NATURE, 1987, 327 (6124) : 707 - 709
  • [9] A GABA TRANSPORTER OPERATES ASYMMETRICALLY AND WITH VARIABLE STOICHIOMETRY
    CAMMACK, JN
    RAKHILIN, SV
    SCHWARTZ, EA
    [J]. NEURON, 1994, 13 (04) : 949 - 960
  • [10] RELEASE OF ENDOGENOUS EXCITATORY AMINO-ACIDS FROM TURTLE PHOTORECEPTORS
    COPENHAGEN, DR
    JAHR, CE
    [J]. NATURE, 1989, 341 (6242) : 536 - 539