SIZE AND PHYSICAL MAP OF THE CAMPYLOBACTER-JEJUNI CHROMOSOME

被引：31

作者：

NUIJTEN, PJM ^{[1
]}

BARTELS, C ^{[1
]}

BLEUMINKPLUYM, NMC ^{[1
]}

GAASTRA, W ^{[1
]}

VANDERZEIJST, BAM ^{[1
]}

机构：

[1] STATE UNIV UTRECHT,SCH VET MED,INST INFECT DIS & IMMUNOL,DEPT BACTERIOL,POB 80-165,3508 TD UTRECHT,NETHERLANDS

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 21期

关键词：

D O I：

10.1093/nar/18.21.6211

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chromosome of Campylobacter Jejuni is circular and approximately 1700 kb in circumference. The size of the genome was determined by field inversion gel electrophoresis of restriction endonuclease fragments using lambda DNA concatamers and yeast chromosomes to calibrate the size of the fragments. In view of the low (32-35%) G + C content of the campylobacter genome, enzymes that recognizes GC-rich sequences were used. Of the enzymes tested BssHII (G/CGCGC), Nc/Il(CC/CGCG) and Sali (G/TCGAC) appeared to be usable. Hybridization of labeled fragments with two or more fragments from digests with a different restriction enzyme gave the information to order the fragments on the C.jejuni chromosome. The localization on the genome of the flagellin and ribosomal gene clusters was determined. © 1990 Oxford University Press.

引用

页码：6211 / 6214

页数：4

共 25 条

[1]

AUSUBEL FM, 1987, CURR PROTOCOLS MOL B, V1

[2] CLONING AND EXPRESSION OF THE CAMPYLOBACTER-JEJUNI GLYA GENE IN ESCHERICHIA-COLI [J].