SENSITIVE STEREOSPECIFIC ASSAY OF WARFARIN IN PLASMA - REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION USING DIASTEREOISOMERIC ESTERS OF (-)-(1S,2R,4R)-ENDO-1,4,5,6,7,7-HEXACHLOROBICYCLO[2.2.1]-HEPT-5-ENE-2-CARBOXYLIC ACID

被引：13

作者：

CARTER, SR ^{[1
]}

DUKE, CC ^{[1
]}

CUTLER, DJ ^{[1
]}

HOLDER, GM ^{[1
]}

机构：

[1] UNIV SYDNEY, DEPT PHARM, SYDNEY, NSW 2006, AUSTRALIA

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 574卷 / 01期

关键词：

D O I：

10.1016/0378-4347(92)80100-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A stereospecific reversed-phase high-performance liquid chromatographic (HPLC) assay for warfarin in plasma has been developed. The assay involves a rapid, simple clean-up procedure which separates warfarin from plasma constituents and warfarin metabolites. Warfarin enantiomers were assayed as their (-)-(1S,2R,4R)-endo-1,4,5,6,7,7-hexachlorobicyclo[2.2.1]hept-5-ene-2-carboxylic acid diastereoisomeric esters by reversed-phase HPLC. Excellent resolution of the diastereoisomers was achieved in less than 10 min. Sensitivity of the assay was approximately 5-10 ng/ml for each isomer.

引用

页码：77 / 83

页数：7

共 21 条

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