PROTEIN-KINASE IN RAT ADIPOCYTES - INFLUENCE OF ANDROGENIC STATUS AND REGIONAL FAT DISTRIBUTION

被引:15
作者
LACASA, D [1 ]
AGLI, B [1 ]
MUR, B [1 ]
DIEUDONNE, MN [1 ]
GIUDICELLI, Y [1 ]
机构
[1] CTR HOSP POISSY,FAC MED,DEPT BIOCHEM,F-78303 POISSY,FRANCE
关键词
D O I
10.1677/joe.0.1380493
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The effects of castration and testosterone treatment on insulin and phorbol ester (TPA)stimulated lipogenic responses, phorbol dibutyrate-specific binding to protein kinase C (PKC) in the cytosol and the beta-PKC isoform level quantified by immunoblotting were compared in rat fat cells from femoral subcutaneous (SC) and deep intra-abdominal (epididymal) fat deposits. In control rats, the PKC content was lower in SC than in epididymal fat cells. After castration, the difference in PKC content between SC and epididymal fat cells was reduced and restored by testosterone treatment. However, androgenic status failed to modify the PKC content in SC fat cells. The lipogenic response to insulin was also differently regulated by the androgenic status in the two fat deposits. After castration, the response was increased in SC fat cells, while it was blunted in epididymal fat cells. These effects were corrected by testosterone administration. These results demonstrate that, in white adipocytes, PKC is an additional biological parameter which varies according to the anatomical origin of the fat cells. They also provide evidence that PKC is controlled by androgens in vivo and emphasize the regional site specificity of such a control.
引用
收藏
页码:493 / 501
页数:9
相关论文
共 34 条
[1]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[2]   IA BINDING LIGANDS AND CAMP STIMULATE NUCLEAR TRANSLOCATION OF PKC IN LYMPHOCYTES-B [J].
CAMBIER, JC ;
NEWELL, MK ;
JUSTEMENT, LB ;
MCGUIRE, JC ;
LEACH, KL ;
CHEN, ZZ .
NATURE, 1987, 327 (6123) :629-632
[3]  
CASTAGNA M, 1982, J BIOL CHEM, V257, P7847
[4]   INSULIN STIMULATION OF GLUCOSE-METABOLISM IN RAT ADIPOCYTES - POSSIBLE IMPLICATION OF PROTEIN-KINASE-C [J].
CHERQUI, G ;
CARON, M ;
WICEK, D ;
LASCOLS, O ;
CAPEAU, J ;
PICARD, J .
ENDOCRINOLOGY, 1986, 118 (05) :1759-1769
[5]   DIFFERENTIAL MODULATION OF THE ADENYLATE-CYCLASE CYCLIC-AMP STIMULATORY PATHWAY BY PROTEIN-KINASE-C ACTIVATION IN RAT ADIPOSE-TISSUE AND ISOLATED FAT-CELLS - INFLUENCE OF COLLAGENASE DIGESTION [J].
DEMAZANCOURT, P ;
DARIMONT, C ;
GIOT, J ;
GIUDICELLI, Y .
BIOCHEMICAL PHARMACOLOGY, 1991, 42 (09) :1791-1797
[6]   INSULIN AND GLUCOSE MODULATE PROTEIN KINASE-C ACTIVITY IN RAT ADIPOCYTES [J].
DRAZNIN, B ;
LEITNER, JW ;
SUSSMAN, KE ;
SHERMAN, NA .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 156 (01) :570-575
[7]   ESTRADIOL MODULATES PROTEIN KINASE-C ACTIVITY IN THE RAT PITUITARY INVIVO AND INVITRO [J].
DROUVA, SV ;
GORENNE, I ;
LAPLANTE, E ;
RERAT, E ;
ENJALBERT, A ;
KORDON, C .
ENDOCRINOLOGY, 1990, 126 (01) :536-544
[8]   INSULIN, OXYTOCIN, AND VASOPRESSIN STIMULATE PROTEIN KINASE-C ACTIVITY IN ADIPOCYTE PLASMA-MEMBRANES [J].
EGAN, JJ ;
SALTIS, J ;
WEK, SA ;
SIMPSON, IA ;
LONDOS, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (03) :1052-1056
[9]   THE MAJOR ENDOGENOUS BOVINE BRAIN PROTEIN-KINASE-C INHIBITOR IS A HEAT-LABILE PROTEIN [J].
FRASER, ED ;
WALSH, MP .
FEBS LETTERS, 1991, 294 (03) :285-289
[10]  
GARCIASAINZ JA, 1988, EUR J PHARMACOL, V146, P193