MOLECULAR-CLONING OF HUMAN HIPPOCALCIN CDNA AND CHROMOSOMAL MAPPING OF ITS GENE

被引：13

作者：

TAKAMATSU, K ^{[1
]}

KOBAYASHI, M ^{[1
]}

SAITOH, S ^{[1
]}

FUJISHIRO, M ^{[1
]}

NOGUCHI, T ^{[1
]}

机构：

[1] TOMY SEIKO CORP,DIV RES & DEV,NERIMA KU,TOKYO 176,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 200卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.1491

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a cDNA clone encoding human hippocalcin from a human hippocampus cDNA library. This clone (hHLP1) consists of 840 nucleotides, including the entire open reading frame of 582 nucleotides, 10 nucleotides of the 5' leader and 248 nucleotides of the 3' noncoding regions. Comparison of the human hippocalcin sequence with the corresponding rat sequence revealed an amino acid identity of 100% and nucleotide identity of 92%. Northern blot analysis showed that a single transcript at a position corresponding to 2.0 kb was detected only in the brain. The human hippocalcin gene was mapped to chromosome 1 by amplification of a human hippocalcin-specific DNA fragment on DNA from human-rodent somatic cell hybrids by using the polymerase chain reaction. (C) 1994 Academic Press, Inc.

引用

页码：606 / 611

页数：6

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