AUTOMATIC PROCEDURE FOR MICROESTIMATION OF 2,3-DIPHOSPHOGLYCERATE

被引：50

作者：

GRISOLIA, S

MOORE, K

LUQUE, J

GRADY, H

机构：

[1] Department of Biochemistry, University of Kansas, University of Kansas Medical Center, Kansas City

[2] Department of Pathology, University of Kansas, University of Kansas Medical Center, Kansas City

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 31卷 / 1-3期

关键词：

D O I：

10.1016/0003-2697(69)90262-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, rapid, and reproducible automated method for the microestimation of 2,3-diphosphoglycerate is presented. The method allows the estimation of 2,3-diphosphoglycerate in dilutions of normal blood of the order of 1:1000 and below; it should be useful in clarifying the relation of 2,3-diphosphoglycerate to oxygen affinity and changes of this important intermediate in diseases such as sickle-cell anemia. Recoveries and normal values of 2,3-diphosphoglycerate in packed red cells and in whole blood are presented. A practical simple procedure to maintain autoanalyzers free of obstructions due to protein precipitation is described. © 1969.

引用

页码：235 / &

共 14 条

[1]

BARTLETT GR, 1959, J BIOL CHEM, V234, P466

[2] EFFECT OF ORGANIC PHOSPHATES FROM HUMAN ERYTHROCYTE ON ALLOSTERIC PROPERTIES OF HEMOGLOBIN [J].