FUNCTIONAL EVIDENCE FOR AN RNA TEMPLATE IN TELOMERASE

被引：337

作者：

SHIPPENLENTZ, D ^{[1
]}

BLACKBURN, EH ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,DEPT MOLEC & CELL BIOL,BERKELEY,CA 94720

来源：

SCIENCE | 1990年 / 247卷 / 4942期

关键词：

D O I：

10.1126/science.1689074

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The RNA moiety of the ribonucleoprotein enzyme telomerase from the ciliate Euplotes crassus was identified and its gene was sequenced. Functional analysis, in which oligonucleotides complementary to portions of the telomerase RNA were tested for their ability to prime telomerase in vitro, showed that the sequence 5′ CAAAACCCCAAA 3′ in this RNA is the template for synthesis of telomeric TTTGGGG repeats by the Euplotes telomerase. The data provide a direct demonstration of a template function for a telomerase RNA and demarcate the outer boundaries of the telomeric template. Telomerase can now be defined as a specialized reverse transcriptase.

引用

页码：546 / 552

页数：7

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