MICROASSAY FOR DNA METHYLTRANSFERASE

被引：55

作者：

ADAMS, RLP

RINALDI, A

SEIVWRIGHT, C

机构：

[1] Department of Biochemistry, University of Glasgow, Glasgow

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1991年 / 22卷 / 01期

基金：

英国医学研究理事会;

关键词：

DNA; METHYLTRANSFERASE; METHYLASE; MICROASSAY;

D O I：

10.1016/0165-022X(91)90077-A

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A microassay for DNA methylase is described which can detect activity in as few as 50 tissue culture cells. The cells are lysed and incubated for 2 h at 37-degrees-C with 3-mu-Ci high specific activity [H-3]AdoMet and 0.5-mu-g poly]d(I-C).d(I-C)] in a volume of 23-mu-1. Ribonuclease is present during the assay and the product DNA is isolated by phenol extraction after protease digestion.

引用

页码：19 / 22

页数：4

共 6 条

[1] DNA METHYLATION - THE EFFECT OF MINOR BASES ON DNA PROTEIN INTERACTIONS
ADAMS, RLP
[J]. BIOCHEMICAL JOURNAL, 1990, 265 (02) : 309 - 320
[2] MOUSE ASCITES DNA METHYLASE - CHARACTERIZATION OF SIZE, PROTEOLYTIC BREAKDOWN AND NUCLEOTIDE RECOGNITION
ADAMS, RLP
GARDINER, K
RINALDI, A
BRYANS, M
MCGARVEY, M
BURDON, RH
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 868 (01) : 9 - 16
[3] BOLDEN A, 1984, J BIOL CHEM, V259, P2437
[4] MONK M, 1990, UNPUB DECREASE DNA M
[5] PREFERENTIAL BINDING OF DNA METHYLTRANSFERASE AND INCREASED DE NOVO METHYLATION OF DEOXYINOSINE CONTAINING DNA
PFEIFER, GP
DRAHOVSKY, D
[J]. FEBS LETTERS, 1986, 207 (01) : 75 - 78
[6] DNA METHYLASE - PURIFICATION FROM ASCITES-CELLS AND EFFECT OF VARIOUS DNA SUBSTRATES ON ITS ACTIVITY
TURNBULL, JF
ADAMS, RLP
[J]. NUCLEIC ACIDS RESEARCH, 1976, 3 (03) : 677 - 695

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