DISTRIBUTION OF CEREBROSPINAL-FLUID OLIGOCLONAL IGM BANDS IN NEUROLOGICAL DISEASES - A COMPARISON BETWEEN AGAROSE ELECTROPHORESIS AND ISOELECTRIC-FOCUSING

被引：9

作者：

SHARIEF, MK ^{[1
]}

THOMPSON, EJ ^{[1
]}

机构：

[1] INST NEUROL, DEPT CLIN NEUROCHEM, LONDON WC1N 3BG, ENGLAND

来源：

JOURNAL OF THE NEUROLOGICAL SCIENCES | 1992年 / 109卷 / 01期

关键词：

IGM; OLIGOCLONAL BANDS; CEREBROSPINAL FLUID; AGAROSE GEL ELECTROPHORESIS; ISOELECTRIC FOCUSING; MULTIPLE SCLEROSIS;

D O I：

10.1016/0022-510X(92)90098-6

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Isoelectric focusing (IEF) and agarose gel electrophoresis were used to detect oligoclonal IgM bands in cerebrospinal fluid and serum samples from 850 patients with diverse neurological diseases. Oligoclonal IgM bands in cerebrospinal fluid were mainly detected in patients with infectious and inflammatory disorders of the nervous system. Both IEF and agarose electrophoresis revealed similar frequencies of oligoclonal IgM bands. Bands detected by IEF were mainly seen in the anodal range. Despite higher resolving capacity, IEF was less specific than agarose gel electrophoresis. It is concluded that oligoclonal IgM bands have important diagnostic significance and that agarose gel electrophoresis is more suitable for their detection in routine clinical work and use in differential diagnosis.

引用

页码：83 / 87

页数：5

共 14 条

[1] CEREBROSPINAL-FLUID OLIGOCLONAL IGM IN MULTIPLE-SCLEROSIS - ANALYTICAL PROBLEMS AND CLINICAL LIMITATIONS [J].

GILES, PD ;

WROE, SJ .

ANNALS OF CLINICAL BIOCHEMISTRY, 1990, 27 :199-207

[2]

HARE DL, 1978, ARCH BIOCHEM BIOPHYS, V187, P274, DOI 10.1016/0003-9861(78)90034-6

[3]

HISCHE EAH, 1988, CLIN CHEM, V34, P665

[4] OLIGOCLONAL IMMUNOGLOBULIN-M IN CEREBROSPINAL-FLUID FROM MULTIPLE-SCLEROSIS PATIENTS [J].

KEIR, G ;

WALKER, RWH ;

THOMPSON, EJ .

JOURNAL OF THE NEUROLOGICAL SCIENCES, 1982, 57 (2-3) :281-285

[5]

LAURENZI MA, 1978, ACTA NEUROL SCAND, V58, P148

[6] INTRATHECAL SYNTHESIS OF FREE IMMUNOGLOBULIN LIGHT-CHAINS AND IGM IN INITIAL MULTIPLE-SCLEROSIS [J].

LOLLI, F ;

SIRACUSA, G ;

AMATO, MP ;

FRATIGLIONI, L ;

DALPOZZO, G ;

GALLI, E ;

AMADUCCI, L .

ACTA NEUROLOGICA SCANDINAVICA, 1991, 83 (04) :239-243

[7]

MCDONALD WI, 1979, BR MED B, V33, P4

[8]

OLSSON T, 1984, CLIN CHEM, V30, P1246

[9] ISOELECTRIC-FOCUSING OF HEPARIN - EVIDENCE FOR COMPLEXING WITH CARRIER AMPHOLYTES [J].

RIGHETTI, PG ;

GIANAZZA, E .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 532 (01) :137-146

[10] GLUTARALDEHYDE-ENHANCED IMMUNOFIXATION - A SENSITIVE NEW METHOD FOR DETECTING OLIGOCLONAL IMMUNOGLOBULIN-M [J].

SHARIEF, MK ;

KEIR, G ;

THOMPSON, EJ .

JOURNAL OF NEUROIMMUNOLOGY, 1989, 23 (02) :149-156

← 1 2 →