PRESENCE OF FUNCTIONALLY DIFFERENT COMPARTMENTS OF THE CA2+ STORE IN SINGLE INTESTINAL SMOOTH-MUSCLE CELLS

被引：46

作者：

YAMAZAWA, T ^{[1
]}

IINO, M ^{[1
]}

ENDO, M ^{[1
]}

机构：

[1] UNIV TOKYO, FAC MED, DEPT PHARMACOL, BUNKYO KU, TOKYO 113, JAPAN

来源：

FEBS LETTERS | 1992年 / 301卷 / 02期

关键词：

CALCIUM STORAGE; CAFFEINE; INOSITOL TRISPHOSPHATE; RYANODINE; CARBACHOL;

D O I：

10.1016/0014-5793(92)81243-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Studies in smooth muscle bundles have shown the presence of functionally different compartments of Ca2+ store, one (S-alpha) sensitive to both caffeine and inositol 1,4,5-trisphosphate (IP3), and the other (S-beta) sensitive only to IP3. Ca2+ release in isolated single smooth muscle cells from guinea pig taenia caeci was studied to see if both compartments exist within a cell. Responses to caffeine and carbachol were consistently observed but were abolished after treatment with ryanodine, while intracellular application of IP3 induced Ca2+ release after the treatment, albeit smaller in size than control. Thus S-alpha and S-beta coexist in a single smooth muscle cell and agonist-induced Ca2+ release requires whole store to be loaded with Ca2+.

引用

页码：181 / 184

页数：4

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