A GENERALIZED-APPROACH TO DETECTION OF ORGANOPHOSPHATE RESISTANCE IN MOSQUITOS

被引：10

作者：

BROGDON, WG

BEACH, RF

BARBER, AM

CORDONROSALES, C

机构：

[1] Malaria Branch, Division of Parasitic Diseases, Center for Infectious Diseases, Centers for Disease Control, Atlanta, Georgia

[2] Centro de Investigaciones en Enfermedades Tropicales, Universidad del Valle de Guatemala, Guatemala City

来源：

MEDICAL AND VETERINARY ENTOMOLOGY | 1992年 / 6卷 / 02期

关键词：

ANOPHELES-ALBIMANUS; ANOPHELES-ARABIENSIS; AN-STEPHENSI; MALATHION; FENITROTHION; ORGANOPHOSPHATE RESISTANCE; BIOASSAY; ENZYME-SPECIFIC ASSAY; MICROTIRE PLATE ASSAY; RESISTANCE DETECTION; MALARIA CONTROL;

D O I：

10.1111/j.1365-2915.1992.tb00585.x

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Insecticide bioassays and biochemical microtitre assays were compared for detection of resistance to the organophosphate insecticides malathion and fenitrothion, using inbred laboratory strains of malaria vectors Anopheles albimanus Wiedemann, An.arabiensis Patton and An.stephensi Liston. With susceptible mosquitoes, the LT100 values determined from bioassays corresponded closely with times taken to abolish the activity of acetylcholinesterase activity in biochemical assays: approximately 2 h for malathion and 3 h for fenitrothion. Resistant strains of all three anophelines showed longer survival correlated with prolonged acetylcholinesterase activity. An.albimanus strains with insensitive acetylcholinesterase survived bioassays with discriminating doses of 1 h exposure to 5% malathion or 1% fenitrothion and were judged as resistant. It is concluded that enzyme-specific microassays provide a reliable means of detecting resistant individuals, with practical advantages over bioassays which do not reveal the resistance mechanism and require large numbers of healthy mosquitoes.

引用

页码：110 / 114

页数：5

共 21 条

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[2]

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