HOST EFFECTS ON GLUTATHIONE-S-TRANSFERASE ACTIVITY IN FASCIOLA-HEPATICA

被引：12

作者：

MILLER, CMD

HOWELL, MJ

BORAY, JC

机构：

[1] NEW S WALES DEPT AGR, ELIZABETH MACARTHUR AGR INST, PRIVATE MAIL BAG 8, CAMDEN, NSW 2570, AUSTRALIA

[2] AUSTRALIAN NATL UNIV, FAC SCI, DIV BIOCHEM & MOLEC BIOL, CANBERRA, ACT 2601, AUSTRALIA

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1993年 / 23卷 / 08期

关键词：

FASCIOLA-HEPATICA; HOST EFFECT; GLUTATHIONE S-TRANSFERASE; SHEEP; CATTLE; MICE; RATS;

D O I：

10.1016/0020-7519(93)90129-M

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Glutathione S-transferases (GST, E.C. 2.5.1.18) in Fasciola hepatica from sheep were previously found to be extremely variable with regard to specific GST activity and isoenzyme profile within and between parasite isolates. The effect of the host on GST activity and isoenzyme profile was examined by infecting mice, rats and cattle as well as sheep with one or the other of two isolates-either salicylanilide-resistant or salicylanilide-susceptible F. hepatica. In the case of both isolates, GST activity in hosts relatively resistant to reinfection-rats and cattle-was lower and more restricted in range compared with hosts susceptible to multiple infection-mice and sheep. In the case of the rat flukes, there was little variation in isozyme profiles whereas cattle flukes appeared to exhibit more variation than sheep flukes. In mice, despite the apparent variability in GST activity, only one GST band was found in the isoenzyme profiles. Therefore, the host appears to exert a pronounced effect on the activity and expression of GSTs in F. hepatica which may be related to variation in the immune responses of the different hosts during infection.

引用

页码：1073 / 1076

页数：4

共 15 条

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