A COMPARISON BETWEEN MELANOTIC AND AMELANOTIC RETINAL-PIGMENT EPITHELIAL-CELLS IN-VITRO CONCERNING THE EFFECTS OF L-DOPA AND OXYGEN ON CELL-CYCLE

被引：24

作者：

AKEO, K ^{[1
]}

TANAKA, Y ^{[1
]}

OKISAKA, S ^{[1
]}

机构：

[1] KEIO UNIV,SCH MED,DEPT OPHTHALMOL,SHINJUKU KU,TOKYO 160,JAPAN

来源：

PIGMENT CELL RESEARCH | 1994年 / 7卷 / 03期

关键词：

OXYGEN; DOPA; CELL CYCLE; PIGMENT EPITHELIUM OF THE EYE;

D O I：

10.1111/j.1600-0749.1994.tb00042.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Melanin precursors and free radicals, cytotoxic substances, are produced during melanin synthesis by tyrosinase. We compared these cytotoxic effects of L-dopa and oxygen on the cell cycle of melanotic retinal pigment epithelial (RPE) cells with amelanotic RPE cells because of the differences of tyrosinase activities between melanotic and amelanotic RPE cells. Flow cytometric DNA analysis of RPE cells exposed to L-dopa (100 muM and 250 muM) were conducted at several oxygen concentrations (20%, 10%, and 5%). The dose-dependent effect of L-dopa to arrest the cell cycle (the S phase) was more pronounced in melanotic than in amelanotic RPE cells, and oxygen caused arrest in the G1 phase.

引用

页码：145 / 151

页数：7

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