DETERMINATION OF HEXOSAMINITOLS BY ION-EXCHANGE CHROMATOGRAPHY AND ITS APPLICATION TO ALKALI-LABILE GLYCOSIDIC LINKAGES IN GLYCOPROTEINS

被引：65

作者：

WEBER, P

WINZLER, RJ

机构：

[1] Department of Biochemistry, Faculty of Health Sciences, State University of New York at Buffalo

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 129卷 / 02期

关键词：

D O I：

10.1016/0003-9861(69)90211-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure is described for the quantitative determination of galactosaminitol, glucosaminitol, and their parent hexosamines by use of the automatic amino acid analyzer using cation-exchange chromatography with a borate-citrate buffer system. The method was used to study the conversion of hexosamines to hexosaminitols in several glycoproteins by alkaline borohydride under different conditions. The amount of galactosamine involved in the O-glycosidic linkage to serine and threonine in different mucins was found to be variable. In bovine, canine, and porcine submaxillary mucin 90-96% of the galactosamine lost during alkaline borohydride treatment was recovered as galactosaminitol. © 1969.

引用

页码：534 / &

共 19 条

[1] ANDERSON B, 1965, J BIOL CHEM, V240, P156
[2] ANDERSON BB, 1967, J BIOL CHEM, V242, P3373
[3] BERTOLIN.M, 1967, J BIOL CHEM, V242, P3776
[4] CARLSON DM, 1966, J BIOL CHEM, V241, P2984
[5] CRIMMIN WRC, 1957, J CHEM SOC, P2838
[6] SEPARATION OF GLUCOSAMINE AND GALACTOSAMINE AND THEIR GLYCITOLS ON AN AMINO ACID ANALYSER
DONALD, ASR
[J]. JOURNAL OF CHROMATOGRAPHY, 1968, 35 (01): : 106 - &
[7] GREGORY JD, 1965, TECHNICON S, P620
[8] LINKAGE BETWEEN SUGARS + AMINO ACIDS IN OVINE SUBMAXILLARY GLAND MUCOPROTEIN OSM
HARBON, S
CLAUSER, H
HERMAN, G
JOLLES, P
ROSSIGNOL, B
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1964, 17 (01) : 57 - &
[9] HOWE C, 1957, P SOC EXP BIOL MED, V96, P88, DOI 10.3181/00379727-96-23401
[10] KATHAN RH, 1967, J BIOL CHEM, V242, P1716

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