SEPARATION AND QUANTITATION OF NICERGOLINE AND RELATED SUBSTANCES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ATMOSPHERIC-PRESSURE IONIZATION MASS-SPECTROMETRY

被引：10

作者：

BANNO, K

HORIMOTO, S

机构：

[1] Analytical Chemistry Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka, 532, 16-89, Kashima 3-chome, Yodogawa-ku

来源：

CHROMATOGRAPHIA | 1991年 / 31卷 / 1-2期

关键词：

COLUMN LIQUID CHROMATOGRAPHY MASS SPECTROMETRY; ATMOSPHERIC PRESSURE IONIZATION; SELECTIVE ION MONITORING; NICERGOLINE; SEMISYNTHETIC ALKALOIDS;

D O I：

10.1007/BF02290495

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study demonstrated the utility of high-performance liquid chromatography/atmospheric pressure ionization mass spectrometry (HPLC/API-MS) in the investigation of 10-alpha-methoxy-1,6-dimethylergoline-8-beta-methanol 5-bromonicotinic acid ester (Nicergoline) and its related substances. The analysis was performed by using an ODS column with ammonium acetate and methanol mixture as the mobile phase. Nicergoline and its related compounds could be characterized by HPLC/API-MS in terms of their molecular weight. The use of multiple ion detection techniques for the quantitation of these compounds was also investigated. The detection limits of nicergoline and its related substances were 5 to 10 ng each at a signal-to-noise ratio of 4. The method was also applied to the study of the decomposition products of nicergoline in simulated gastric and intestinal fluids.

引用

页码：50 / 54

页数：5

共 22 条

[1]

ANDRIES P, 1987, ANAL CHEM, V59, P2647

[2] STUDIES ON METABOLISM OF ERGOLINE DERIVATIVES - METABOLISM OF NICERGOLINE IN MAN AND IN ANIMALS [J].

ARCAMONE, F ;

MINGHETT.A ;

NICOLELL.V ;

GRAFNETT.J ;

GLASSER, AG .

BIOCHEMICAL PHARMACOLOGY, 1972, 21 (16) :2205-&

[3]

ARCARI G, 1968, BRIT J PHARMACOL, V34, pP700

[4]

BANNO K, 1989, IYAKUHIN KENKYU, V20, P621

[5] ERGOLINE DERIVATIVES .9. CONFIGURATION AND CONFORMATION OF 10-METHOXYDIHYDROLYSERGIC ACID DERIVATIVES [J].

BARBIERI, W ;

BERNARDI, L ;

BOSISIO, G ;

TEMPERILLI, A .

TETRAHEDRON, 1969, 25 (11) :2401-+

[6]

BERNARDI L, 1964, GAZZ CHIM ITAL, V95, P384

[7] ATMOSPHERIC-PRESSURE IONIZATION MASS-SPECTROMETRY - CORONA DISCHARGE ION-SOURCE FOR USE IN LIQUID CHROMATOGRAPH MASS SPECTROMETER-COMPUTER ANALYTICAL SYSTEM [J].

CARROLL, DI ;

DZIDIC, I ;

STILLWELL, RN ;

HAEGELE, KD ;

HORNING, EC .

ANALYTICAL CHEMISTRY, 1975, 47 (14) :2369-2373

[8]

COVEY TR, 1986, ANAL CHEM, V58, P1451

[9] HIGH-SPEED LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY FOR THE DETERMINATION OF DRUGS IN BIOLOGICAL SAMPLES [J].

COVEY, TR ;

LEE, ED ;

HENION, JD .

ANALYTICAL CHEMISTRY, 1986, 58 (12) :2453-2460

[10]

ECKERS C, 1982, Analytical Proceedings, V19, P133

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