BRADYKININ MODULATES MUCIN SECRETION BUT NOT SYNTHESIS FROM AN INTESTINAL GOBLET CELL-LINE

被引:9
作者
STANLEY, CM [1 ]
PHILLIPS, TE [1 ]
机构
[1] UNIV MISSOURI,DIV BIOL SCI,COLUMBIA,MO 65211
来源
AGENTS AND ACTIONS | 1994年 / 42卷 / 3-4期
关键词
BRADYKININ; MUCIN; MUCUS; HT29; GOBLET CELL;
D O I
10.1007/BF01983480
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The effect of the inflammatory mediator bradykinin on glycoprotein synthesis and mucin secretion in the human colonic adenocarcinoma cell line HT29-18N2 was examined. Bradykinin, at a threshold of 0.01 mu M, accelerated the rate of mucin discharge as assessed by a mucin-specific ELISA. Using immunofluorescence microscopy, a thick meshwork of extracellular mucus was observed over bradykinin-treated monolayers but not mock-treated controls. Morphometric analysis of bradykinin-treated monolayers revealed no decreases in intracellular mucin stores or any other easily discernable morphological alteration. The ability of the cyclooxygenase inhibitors indomethacin and naproxen to decrease the response to bradykinin by approximately 68% indicates the effect is mediated, at least partially, through the generation of prostaglandins. Bradykinin did not alter the rate of incorporation of H-3-glucosamine into newly synthesized glycoproteins. Bradykinin-accelerated mucin secretion may be linked to the depletion of intracellular mucin stores in the inflammatory bowel disease ulcerative colitis.
引用
收藏
页码:141 / 145
页数:5
相关论文
共 31 条
  • [1] ENHANCEMENT OF KALLIKREIN PRODUCTION AND KININ SENSITIVITY IN T84-CELLS BY GROWTH IN THE NUDE-MOUSE
    BAIRD, AW
    MILLER, DH
    SCHWARTZ, DA
    MARGOLIUS, HS
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 261 (05): : C822 - C827
  • [2] BAKER AP, 1977, AM REV RESPIR DIS, V115, P811
  • [3] IBUPROFEN AUGMENTS BRADYKININ-INDUCED GLYCOCONJUGATE SECRETION BY HUMAN NASAL-MUCOSA INVIVO
    BARANIUK, JN
    SILVER, PB
    KALINER, MA
    BARNES, PJ
    [J]. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1992, 89 (05) : 1032 - 1039
  • [4] KININS INDUCE RAPID STRUCTURAL-CHANGES IN COLON CONCOMITANT WITH CHLORIDE SECRETION
    BARON, DA
    MILLER, DH
    MARGOLIUS, HS
    [J]. CELL AND TISSUE RESEARCH, 1986, 246 (03) : 589 - 594
  • [5] FIBROBLASTS MODULATE INTESTINAL SECRETORY RESPONSES TO INFLAMMATORY MEDIATORS
    BERSCHNEIDER, HM
    POWELL, DW
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1992, 89 (02) : 484 - 489
  • [6] KININ EFFECTS ON ELECTROGENIC ION-TRANSPORT IN PRIMARY CULTURES OF PIG RENAL PAPILLARY COLLECTING TUBULE CELLS
    CUTHBERT, AW
    GEORGE, AM
    MACVINISH, L
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1985, 249 (03): : F439 - F447
  • [7] KININS STIMULATE NET CHLORIDE SECRETION BY THE RAT COLON
    CUTHBERT, AW
    MARGOLIUS, HS
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 1982, 75 (04) : 587 - 598
  • [8] KININ EFFECTS ON ION-TRANSPORT IN MONOLAYERS OF HCA-7 CELLS, A LINE FROM A HUMAN COLONIC ADENOCARCINOMA
    CUTHBERT, AW
    KIRKLAND, SC
    MACVINISH, LJ
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 1985, 86 (01) : 3 - 5
  • [9] CHARACTERIZATION OF A B2-BRADYKININ RECEPTOR IN RAT RENAL MESANGIAL CELLS
    EMOND, C
    BASCANDS, JL
    PECHER, C
    CABOSBOUTOT, G
    PRADELLES, P
    REGOLI, D
    GIROLAMI, JP
    [J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 1990, 190 (03) : 381 - 392
  • [10] LECTIN BINDING PATTERNS TO PLASMALEMMAL GLYCOCONJUGATES OF GOBLET CELLS UNDERGOING DIFFERENTIATION INVITRO
    FRISCH, EB
    PHILLIPS, TE
    [J]. JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE, 1990, 16 (01): : 25 - 36