SERIAL PROPAGATION AND PURIFICATION OF CHICKEN ANEMIA AGENT IN MDCC-MSB1 CELL-LINE

被引：71

作者：

GORYO, M ^{[1
]}

SUWA, T ^{[1
]}

MATSUMOTO, S ^{[1
]}

UMEMURA, T ^{[1
]}

ITAKURA, C ^{[1
]}

机构：

[1] TOTTORI UNIV, FAC AGR, DEPT VET PATHOL, TOTTORI 680, JAPAN

来源：

AVIAN PATHOLOGY | 1987年 / 16卷 / 01期

关键词：

D O I：

10.1080/03079458708436360

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The TK-5803 strain of an agent which induces chicken anaemia was propagated and titrated in cultures of the MDCC-MSB1 cell line. When the isolates (tentatively designated MSB1-TK-5803 strain) from the original material were inoculated into 1-day-old susceptible chicks, they showed a severe pathogenicity, inducing anaemia with aplasia of the bone marrow and atrophy of the lymphoid organs. On density gradient separation, the peak of their infectivity titre appeared at a density of 1.35 to 1.36 g/cm3 and numerous virus particles were demonstrated in the same fraction. The agent was resistant to heating for 30 min at 60.degree. C and 50% of chloroform for 15 min at 4.degree. C, and passed through a 25 nm membane filter, but it was completely inactivated after heating for 30 min at 70.degree. C or more. The purified virus particles were spherical or hexagonal in shape and 19.1 .+-. 0.2 nm in diameter.

引用

页码：149 / +

页数：1

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