CARBAMYL PHOSPHATE-DEPENDENT ATP SYNTHESIS CATALYZED BY FORMYLTETRAHYDROFOLATE SYNTHETASE

被引：9

作者：

BUTTLAIRE, DH ^{[1
]}

BALFE, CA ^{[1
]}

WENDLAND, MF ^{[1
]}

HIMES, RH ^{[1
]}

机构：

[1] UNIV KANSAS,DEPT BIOCHEM,LAWRENCE,KS 66045

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 567卷 / 02期

关键词：

ATP synthesis; Carbamyl phosphate; Formyltetrahydrofolate synthetase;

D O I：

10.1016/0005-2744(79)90131-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Formyltetrahydrofolate synthetase (formate:tetrahydrofolate ligase (ADP-forming), EC 6.3.4.3) from Clostridium cylindrosporum catalyzes phosphate transfer from carbamyl phosphate to ADP. This activity is lost when monovalent cations are removed and is recovered when K+ is added back. Carbamyl phosphate is an inhibitor of the formyltetrahydrofolate synthetase forward reaction, and formate as well as phosphate inhibit the ATP synthesis reaction. Acetyl phosphate and phosphonoacetate are inhibitors of both reactions. The results of kinetic studies support the concept that carbamyl phosphate is an analog of the putative intermediate of the formyltetrahydrofolate synthetase reaction, formyl phosphate. © 1979.

引用

页码：453 / 463

页数：11

共 15 条

[1]

BUTTLAIRE DH, 1975, J BIOL CHEM, V250, P261

[2]

BUTTLAIRE DH, 1976, J BIOL CHEM, V251, P4159

[3]

BUTTLAIRE DH, 1975, J BIOL CHEM, V250, P254

[4]

CLELAND WW, 1967, ADV ENZYMOL RAMB, V29, P1

[5]

CURTHOYS NP, 1972, J BIOL CHEM, V247, P1965

[6]

CURTHOYS NP, 1971, J BIOL CHEM, V246, P6942

[7]

Himes R H, 1973, CRC Crit Rev Biochem, V1, P501, DOI 10.3109/10409237309105441

[8]

PINCHOT GB, 1957, J BIOL CHEM, V229, P11

[9]

POLAKIS SE, 1972, J BIOL CHEM, V247, P1335

[10]

RABINOWITZ JC, 1957, J BIOL CHEM, V229, P321

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