CHEMOTACTIC FACTOR FOR TUMOR-CELLS DERIVED FROM THE C5A FRAGMENT OF COMPLEMENT COMPONENT C5

被引：38

作者：

ORR, W ^{[1
]}

PHAN, SH ^{[1
]}

VARANI, J ^{[1
]}

WARD, PA ^{[1
]}

KREUTZER, DL ^{[1
]}

WEBSTER, RO ^{[1
]}

HENSON, PM ^{[1
]}

机构：

[1] NATL JEWISH HOSP & RES CTR,DEPT PEDIAT,DENVER,CO 80206

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 04期

关键词：

D O I：

10.1073/pnas.76.4.1986

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Previously, we have established that the 5th component of complement (C5) serves as an important source of mediators that have locomotory (chemotactic) activity for leukocytes and tumor cells. C5a, a fragment (Mr11,200) derived from the NH 2-terminal portion of the α chain of C5, is the major chemotactic peptide for leukocytes. The present studies demonstrate that cleavage of C5a with trypsin generates a derivative peptide that is chemotactic for tumor cells (Walker carcino-sarcoma). This fragment has an estimated Mr of 6000 as assessed by gel filtration and does not require the COOH-terminal arginine of C5a, because equivalent amounts of chemotactic activity for tumor cells can be generated from des-Arg-C5a by digestion with trypsin. The C5a-derived chemotactic peptide for tumor cells demonstrates peak activity at approximately 1 pM. These studies emphasize the key role of the C5a region of the C5 molecule in the generation of peptides that affect locomotory responses.

引用

页码：1986 / 1989

页数：4

共 15 条

[1] FLUOROMETRIC ASSAY OF PROTEINS IN NANOGRAM RANGE [J].