REVERSIBILITY OF NA+/MG2+ ANTIPORT IN RAT ERYTHROCYTES

被引：39

作者：

GUNTHER, T

VORMANN, J

机构：

[1] Institute of Molecular Biology and Biochemistry, Free University of Berlin, D-14195 Berlin

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1995年 / 1234卷 / 01期

关键词：

SODIUM ION; MAGNESIUM ION; ANTIPORT; AMILORIDE; PROTEIN KINASE C; (RAT ERYTHROCYTE);

D O I：

10.1016/0005-2736(94)00267-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rat erythrocytes loaded with Mg2+ plus Na+ performed Mg2+ uptake under an intracellular/extracellular Na+ gradient. Mg2+ uptake was coupled to Na+ release at a stoichiometric ratio of 1 Mg2+/2 Na+. Mg2+ uptake was inhibited by amiloride, imipramine and quinidine. Mn2+ was taken up by the same transporter as Mg2+. Similar results had been found for net Mg2+ efflux via Na+/Mg2+ antiport in such rat erythrocytes. Hence, it can be concluded that Na+/Mg2+ antiport in Mg2+-loaded rat erythrocytes operates reversibly according to the direction of the Na+ gradient which is a contributing driving force. Net Mg2+ influx was dependent on ATP which increased the affinity of intracellular Mg2+ by activating Na+/Mg2+ antiport. Mg2+ uptake was increased by phorbol ester and inhibited by staurosporine, indicating that ATP may function via protein phosphorylation by protein kinase C.

引用

页码：105 / 110

页数：6

共 22 条

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