MEASUREMENT OF SERUM STEROID AND GONADOTROPIN-LEVELS AND UTERINE AND OVARIAN VARIABLES THROUGHOUT 4 DAY AND 5 DAY ESTROUS CYCLES IN THE RAT

The present study was undertaken to characterize hormonal and morphological events in 4 day and 5 day rat cycles, in order to test 3 different theories concerning the etiological factors altering follicular phase length in mammals. Three rats were killed at each 2 h interval throughout each cycle type. In addition to organ weight measurements, serum levels of estradiol, progesterone, 20alpha-hydroxypreg-4-en-3-one, LH, FSH and prolactin were assessed in terminal samples. The beginning of uterine intraluminal fluid accumulation coincided with the decline in progesterone levels seen during diestrus, rather than with the rise in estrogen and was delayed 12 h in the 5 day cycle from the time of last estrus. The retention of this fluid during the evening of proestrus was 6 h longer in the 4 day cycling rat. Ovulation occurred earlier in the 5 day cycling rats than in those with a 4 day cycle. No significant differences in the gonadotropins of 20alpha-hydroxypreg-4-en-3-one were seen when comparing the 2 types of cycles. Estradiol rose during the night of diestrus I (the same time in the cycle) and was identical in both cycle types. Progesterone was not released on proestrus prior to the LH surge in either type of cycle. However, we did find that progesterone values were higher for a longer period of time throughout diestrus I and 5 day cycles. These data have led us to conclude that the 5 day cycle is due to a prolonged progesterone secretion during the metestrous and diestrous stage of the cycle.