ISOLATION AND CHARACTERIZATION OF 8 TUMOR NECROSIS FACTOR-INDUCED GENE-SEQUENCES FROM HUMAN FIBROBLASTS

被引：177

作者：

LEE, TH

LEE, GW

ZIFF, EB

VILCEK, J

机构：

[1] NYU MED CTR,DEPT MICROBIOL,550 1ST AVE,NEW YORK,NY 10016

[2] NYU MED CTR,DEPT BIOCHEM,NEW YORK,NY 10016

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 05期

关键词：

D O I：

10.1128/MCB.10.5.1982

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A λ cDNA library was prepared from polyadenylated RNA isolated from quiescent human diploid FS-4 fibroblasts stimulated with tumor necrosis factor for 3 h. Differential screening was used to isolate cDNA sequences that are stimulated by tumor necrosis factor. Eight distinct tumor necrosis factor-stimulated gene sequences (designated TSG-1, -6, -8, -12, -14, -21, -27, and -37) were partially sequenced and compared with known sequences from GenBank. TSG-1 was identical to the gene for interleukin-8. TSG-8 corresponded to the gene for monocyte chemotactic and activating factor. TSG-21 and -27 were identical to the genes for collagenase and stromelysin, respectively. The other four sequences showed no homologies with known genes. Patterns of induction of mRNAs corresponding to the eight cloned cDNAs by various cytokines, growth factors, and activators of second messenger pathways were analyzed in FS-4 cells.

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页码：1982 / 1988

页数：7

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