FRACTIONATION OF THE WATER-INSOLUBLE PROTEINS FROM CALF LENS

被引：38

作者：

KIBBELAAR, MA

BLOEMENDAL, H

机构：

[1] Department of Biochemistry, University of Nijmegen, 6525 EZ Nijmegen

来源：

EXPERIMENTAL EYE RESEARCH | 1979年 / 29卷 / 06期

关键词：

D O I：

10.1016/0014-4835(79)90024-1

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

The water-insoluble proteins from calf lens have been subdivided into a urea-soluble (USL) and a urea-insoluble (UIL) part. USL can be fractionated into four components by gel filtration on Sephadex G 200 columns equilibrated in 6 m-urea. One of the fractions contains the major cytoskeletal proteins MP42 and MP55. The major fiber plasma membrane protein MP26 remains in the UIL fraction and can be isolated by preparative SDS-polyacrylamide gel electrophoresis. Peptide mapping reveals that MP26 is definitely different from the major β-crystallin component βBp. © 1979.

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页码：679 / 688

页数：10

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