EVALUATION OF MONOCLONAL-ANTIBODIES AGAINST PLASMODIUM-VIVAX SPOROZOITES FOR ELISA DEVELOPMENT

被引：36

作者：

WIRTZ, RA

CHAROENVIT, Y

BURKOT, TR

ESSER, KM

BEAUDOIN, RL

COLLINS, WE

ANDRE, RG

机构：

[1] Department of Entomology, Walter Reed Army Institute of Research, Washington, District of Columbia

[2] Naval Medical Research Institute, Bethesda, Maryland

[3] Queensland Institute of Medical Research, Brisbane, Queensland

[4] Smith, Kline & French Laboratories, Sweedeland, Pennsylvania

[5] Malaria Branch, Centers for Disease Control, Atlanta, Georgia

[6] Uniformed Services University of the Health Sciences, Bethesda, Maryland

来源：

MEDICAL AND VETERINARY ENTOMOLOGY | 1991年 / 5卷 / 01期

关键词：

MALARIA VECTORS; PLASMODIUM-VIVAX; CIRCUMSPOROZOITE PROTEIN; SPOROZOITE IDENTIFICATION; ELISA;

D O I：

10.1111/j.1365-2915.1991.tb00515.x

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Nine monoclonal antibodies (MAbs) developed against Plasmodium vivax (Grassi & Feletti) salivary gland sporozoites were evaluated for use in an enzyme-linked immunosorbent assay (ELISA), using sporozoites developed in Anopheles dirus Peyton & Harrison An.gambiae Giles and An.maculatus Theobald. Four of the antibodies were unsuitable due to the low sensitivity of the resulting assays or the requirement for high concentrations of capture antibody. An additional two MAbs were rejected because they resulted in assays with high background absorbance, attributed to self-binding. Of the three remaining MAbs, the use of Navy vivax sporozoite (NVS) 3 resulted in an ELISA with the highest sensitivity and the lowest concentration requirement for capture antibody. Assay sensitivity varied with sporozoite strain indicating possible quantitative epitope heterogeneity. None of the MAbs cross-reacted with the heterologous sporozoites tested by immunofluorescence antibody assay (IFA). The IFA activity was not an indicator of ELISA sensitivity. The use of MAb NVS 3 in a standardized ELISA method resulted in an assay 10 times more sensitive than reported previously for P.vivax sporozoites, with a detection limit of fewer than 100 sporozoites per mosquito.

引用

页码：17 / 22

页数：6

共 18 条

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