IDENTIFICATION OF THE GENE FOR THE DEVELOPMENTALLY EXPRESSED 70KDA HEAT-SHOCK PROTEIN (P70) OF MOUSE SPERMATOGENIC CELLS

Mouse spermatogenic cells synthesize a 70-kDa protein (P70) closely related to the major heat-shock protein (hsp70) of mammalian cells (R. L. Allen, D. A. O'Brien, and E. M. Eddy, Mol. Cell. Biol.8, 828-832, 1988). Expression of P70 is developmentally regulated while hsp70 is induced in response to stress, suggesting that P70 is the product of a unique member of the Hsp70 multigene family transcribed in spermatogenic cells. A strong candidate for this gene was the Hsp70.2 gene (Z. F. Zakeri, D. J. Wolgemuth, and C. R. Hunt, Mol. Cell. Biol.8, 2925-2932, 1988). A DNA segment from the 5′ region of Hsp70.2 hybridized to a 2.7-kb transcript with a temporal pattern of expression in mouse spermatogenic cells similar to the P70 protein. We used a polyclonal antiserum generated against a synthetic peptide predicted from the Hsp70.2 sequence to characterize its protein product and to isolate cDNA clones from a pachytene spermatocyte expression library. The antiserum reacted specifically with meiotic and postmeiotic spermatogenic cells on sections of mouse testis. It recognized the P70 protein on Western blots of two-dimensional gels and did not bind to other heat-shock proteins of spermatogenic or somatic cells. The cDNAs hybridized to a 2.7-kb mRNA that was abundant in unstressed pachytene spermatocytes and round spermatids but was not detected in other cell types. Two cDNAs were sequenced and found to be 99% homologous to the 3′ end of the Hsp70.2 gene. These data strongly supported the hypothesis that P70 is the expressed product of the Hsp70.2 gene in mouse spermatogenic cells. © 1992.