ADSORPTION LOSSES DURING EXTRACTION AND DERIVATIZATION EFFICIENCY BY BENZOYLATION OF PLANT PUTRESCINE FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS

被引:12
作者
HAUSCHILD, MZ
机构
[1] Laboratory of Environmental Sciences and Ecology, Technical University of Denmark, DK-2800 Lyngby
来源
JOURNAL OF CHROMATOGRAPHY | 1993年 / 630卷 / 1-2期
关键词
D O I
10.1016/0021-9673(93)80477-P
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The method for the determination analysis of plant polyamines through the reversed-phase HPLC separation of the benzamide derivatives was investigated for putrescine (1,4-diaminobutane) with the purpose of increasing reproducibility without losing speed. Putrescine was found not to adsorb on differential vial materials, but a significant binding to plant material in the extraction step was found. The overall benzoylation efficiency varied between 59 and 83%, and the use of 1,6-diaminohexane as an internal standard is suggested in order to obtain reproducible results.
引用
收藏
页码:397 / 401
页数:5
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