MEMBRANE-ASSOCIATED NADH DEHYDROGENASE-ACTIVITIES IN RHODOBACTER-CAPSULATUS - PURIFICATION OF A DIHYDROLIPOYL DEHYDROGENASE

被引：2

作者：

BERKS, BC ^{[1
]}

MCEWAN, AG ^{[1
]}

FERGUSON, SJ ^{[1
]}

机构：

[1] UNIV OXFORD, DEPT BIOCHEM, S PARKS RD, OXFORD OX1 3QU, ENGLAND

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1993年 / 139卷

关键词：

D O I：

10.1099/00221287-139-8-1841

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The presence of several NADH dehydrogenase activities associated with cytoplasmic membrane vesicles of chemoheterotrophically grown Rhodobacter capsulatus MT1131 was demonstrated by combining isoelectric focusing with NADH-tetranitrobluetetrazolium activity staining, a procedure that should have general applicability in the analysis of bacterial NADH dehydrogenase activities. Low pI (pI = 5.7), Mid pI (pI = 6.9) and High pI (pI = 8.5) bands were resolved. The Mid pI NADH dehydrogenase activity was purified and identified as a dihydrolipoyl dehydrogenase. Our data indicate that this dihydrolipoyl dehydrogenase is derived from a 2-oxoacid dehydrogenase complex which is associated with the cytoplasmic membrane.

引用

页码：1841 / 1851

页数：11

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