DEMONSTRATION OF A SPECIFIC [H-3] INS(1,4,5)P3 BINDING-SITE IN RAT-HEART SARCOPLASMIC-RETICULUM

被引：25

作者：

HUISAMEN, B

MOUTON, R

OPIE, LH

LOCHNER, A

机构：

[1] UNIV STELLENBOSCH, DEPT MED PHYSIOL & BIOCHEM,MRC, PROGRAMME EXPTL BIOL,POB 19063, TYGERBERG 7505, SOUTH AFRICA

[2] UNIV CAPE TOWN, MRC, ISCHAEM HEART DIS UNIT, CAPE TOWN, SOUTH AFRICA

来源：

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY | 1994年 / 26卷 / 03期

关键词：

INOSITOLTRISPHOSPHATE; INOSITOLTETRAKISPHOSPHATE; SARCOPLASMIC RETICULUM;

D O I：

10.1006/jmcc.1994.1043

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Previous studies have suggested that inositol (1,4,5)-trisphosphate (InsP3) might induce release of Ca2+ by sarcoplasmic reticulum (SR) vesicles isolated from heart muscle. In this study binding sites specific for InsP3 in isolated SR vesicles from heart muscle were demonstrated. Scatchard analysis of a binding isotherm indicated two binding sites, viz a high affinity site with a Kd = 20 nM and Bmax = 4.2 pmol/mg protein and a low affinity site with a Kd = 424 nM and Bmax = 31 pmol/mg protein. Specificity of binding was demonstrated by using structural analogues. In addition to the finding of binding sites for InsP3, this membrane preparation also demonstrated a possible binding site for InsP4. © 1994 Academic Press Limited.

引用

页码：341 / 349

页数：9

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