IDENTIFICATION OF THE FUNCTIONAL SUBUNIT OF A DIMERIC TRANSCRIPTION ACTIVATOR PROTEIN BY USE OF ORIENTED HETERODIMERS

被引:67
作者
ZHOU, YH
BUSBY, S
EBRIGHT, RH
机构
[1] RUTGERS STATE UNIV,WAKSMAN INST,NEW BRUNSWICK,NJ 08855
[2] UNIV BIRMINGHAM,SCH BIOCHEM,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND
关键词
D O I
10.1016/0092-8674(93)90236-J
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have constructed heterodimers consisting of two subunits: one CAP subunit that has a nonfunctional activating region but wild-type DNA binding specificity, and one CAP subunit that has a functional activating region but non-wild-type DNA binding specificity. We have oriented the heterodimers on lac promoter DNA by use of promoter derivatives that have DNA sites for CAP consisting of one wild-type half site and one non-wild-type half site, and we have analyzed the abilities of the oriented heterodimers to activate transcription. Our results indicate that transcription activation requires the activating region of only one subunit of CAP: the promoter-proximal subunit. The oriented heterodimers method of this report should be generalizable to other dimeric transcription activator proteins.
引用
收藏
页码:375 / 379
页数:5
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