HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF TAMOXIFEN AND METABOLITES IN PLASMA AND TISSUES

被引：26

作者：

LIM, CK

CHOW, LCL

YUAN, ZX

SMITH, LL

机构：

[1] Mrc Toxicology Unit, University of Leicester, Leicester, LE1 9HN, Hodgkin Building, P.O. Box 138, Lancaster Road

[2] Clinical Biochemistry Laboratory, Princess Margaret Hospital, Kowloon

来源：

BIOMEDICAL CHROMATOGRAPHY | 1993年 / 7卷 / 06期

关键词：

D O I：

10.1002/bmc.1130070606

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An isocratic reversed-phase high performance liquid chromatographic method for the determination of tamoxifen and its metabolites in plasma and tissues is described. Plasma or tissue homogenate was extracted with methanol/dimethyl sulphoxide (4:1 v/v). The supernatant after centrifugation was separated on a BDS-Hypersil column with methanol/0.5 m ammonium acetate (75:25 v/v) as the mobile phase. The recoveries of tamoxifen added to plasma and liver tissue hemogenate by the extraction procedure were 102+/-1.6 and 98+/-2.4% (mean+/-SD, n = 6), respectively. The solutes were detected at 280 nm with a detection limit of 0.25 mug/mL for tamoxifen.

引用

页码：311 / 314

页数：4

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