RAPID AND HIGHLY SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF HISTAMINE AND 3-METHYLHISTAMINE IN BIOLOGICAL SAMPLES USING FLUORESCAMINE AS THE DERIVATIZING AGENT

被引：20

作者：

VANHAASTER, CMCJ

ENGELS, W

LEMMENS, PJMR

HORNSTRA, G

VANDERVUSSE, GJ

机构：

[1] UNIV LIMBURG,DEPT HUMAN BIOL,6200 MD MAASTRICHT,NETHERLANDS

[2] UNIV LIMBURG,DEPT PHYSIOL,6200 MD MAASTRICHT,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 617卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80493-N

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A highly sensitive and rapid high-performance liquid chromatographic assay for the determination of histamine and 3-methyl-histamine in biological samples using 1-methylhistamine as the internal standard is described. Samples were purified and concentrated on cation-exchange columns and derivatized with fluorescamine. The lower detection limit was 20 pg on-column. Linearity was demonstrated up to 20 ng on-column. The samples could be derivatized simultaneously before injection and were stable for 7 days. The method was used for the determination of histamine and related compounds in coronary perfusates, extracts of homogenized rat hearts, and supernatants of stimulated peritoneal mast cells.

引用

页码：233 / 240

页数：8

共 24 条

[1] A DIRECT AND SENSITIVE DETERMINATION OF HISTAMINE IN ACID-DEPROTEINIZED BIOLOGICAL SAMPLES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].