STRUCTURAL-PROPERTIES OF 3.0-KB AND 3.2-KB TRANSCRIPTS ENCODING PLATELET-DERIVED ENDOTHELIAL-CELL GROWTH-FACTOR THYMIDINE PHOSPHORYLASE IN A431-CELLS

被引：15

作者：

USUKI, K ^{[1
]}

GONEZ, LJ ^{[1
]}

WERNSTEDT, C ^{[1
]}

MOREN, A ^{[1
]}

MIYAZONO, K ^{[1
]}

CLAESSONWELSH, L ^{[1
]}

HELDIN, CH ^{[1
]}

机构：

[1] BIOMED CTR,LUDWIG INST CANC RES,S-75124 UPPSALA,SWEDEN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1994年 / 1222卷 / 03期

关键词：

NUCLEOTIDE SEQUENCE; THYMIDINE PHOSPHORYLASE; PLATELET-DERIVED ENDOTHELIAL CELL GROWTH FACTOR; GROWTH FACTOR;

D O I：

10.1016/0167-4889(94)90049-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Platelet-derived endothelial cell growth factor/thymidine phosphorylase (PD-ECGF/TP) is a 90 kDa protein consisting of two non-covalently associated subunits. In addition to the previously identified 1.8 kilobase (kb) PD-ECGF/TP mRNA, the human epidermoid carcinoma cell line A431 was found to express 3.0 kb and 3.2 kb transcripts. cDNA cloning of the larger transcripts revealed that they contain long 5' leader sequences of 1.5 kb and 1.7 kb, respectively, and the same open reading frame encoding PD-ECGF/TP as that of the 1.8 kb transcript. Comparison with the published PD-ECGF/TP genomic sequence shows that the long 5' leader sequence contain 7 of 8 copies of Sp-1 binding sites present in the transcription promoter region of the 1.8 kb transcript.

引用

页码：411 / 414

页数：4

共 14 条

[1] PURIFICATION OF BIOLOGICALLY-ACTIVE GLOBIN MESSENGER-RNA BY CHROMATOGRAPHY ON OLIGOTHYMIDYLIC-ACID-CELLULOSE [J].