NONPULSING REAGENT METERING FOR CONTINUOUS COLORIMETRIC DETECTION SYSTEMS

被引：11

作者：

JOLLEY, RL

PITT, WW

SCOTT, CD

机构：

[1] Oak Ridge National Laboratory, 2 2 Operated for the U. S. Atomic Energy Commission by Union Carbide Corporation., Oak Ridge

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 28卷 / 1-3期

关键词：

D O I：

10.1016/0003-2697(69)90183-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been developed for metering liquid reagents without variations in flow rate for continuous colorimetric detection systems. The reagent is forced from a storage chamber by hydraulic head or by gas overpressure, and flow control is achieved by resistance in the delivery line which may be either capillary tubing and/or a needle valve. Continuous flow of the reagent reaction mixture through the colorimeter is achieved by using a water aspirator. Such a system is now employed routinely in a continuous carbohydrate analyzer in which concentrated sulfuric acid and a 5% phenol solution are continously metered and mixed with the effluent from an anion-exchange resin column that separates the carbohydrates in physiological samples. © 1969.

引用

页码：300 / +

页数：1