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PARTIAL PURIFICATION OF MITOCHONDRIAL AND SUPERNATANT DNA POLYMERASE FROM SACCHAROMYCES CEREVISIAE

被引：28
作者：
IWASHIMA, A
RABINOWITZ, M
机构：
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 178卷 / 02期
关键词：
D O I：
10.1016/0005-2744(69)90396-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Yeast mitochondria incorporate [3H]TMP into DNA that has a buoyant density of mitochondrial DNA. The reaction does not require additional DNA and is not inhibited by deoxyribonuclease. DNA polymerase has been solubilized and purified about 50 fold from mitochondria and about 150 fold from the supernatant fraction of the cell. Enzymatic properties of the two enzyme preparations are similar. Density gradient centrifugation indicates that the supernatant and mitochondrial enzymes have a different molecular size. © 1969.
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页码：283 / +
页数：1
相关论文
共 25 条
[1]  
BESSMAN MJ, 1958, J BIOL CHEM, V233, P171
[2]  
DAVIDSON JN, 1967, PROGRESS NUCLEIC ED, V6, P143
[3]   ISOLATION + PROPERTIES OF INTACT MITOCHONDRIA FROM SPHEROPLASTS OF YEAST [J].
DUELL, EA ;
UTTER, MF ;
INOUE, S .
JOURNAL OF BACTERIOLOGY, 1964, 88 (06) :1762-&
[4]  
Granick S, 1967, Prog Nucleic Acid Res Mol Biol, V6, P143
[5]  
HELGE H, 1965, ARCH EXP PATHOL PH, V251, P44
[6]   BIOCHEMICAL CORRELATES OF RESPIRATORY DEFICIENCY .7. GLUCOSE REPRESSION [J].
JAYARAMAN, J ;
COTMAN, C ;
MAHLER, HR ;
SHARP, CW .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1966, 116 (1-3) :224-+
[7]  
KALF GF, 1968, J BIOL CHEM, V243, P4904
[8]   OXIDATIVE PHOSPHORYLATION IN YEAST .I. ISOLATION AND PROPERTIES OF PHOSPHORYLATING MITOCHONDRIA FROM STATIONARY PHASE CELLS [J].
KOVAC, L ;
BEDNAROVA, H ;
GREKSAK, M .
BIOCHIMICA ET BIOPHYSICA ACTA, 1968, 153 (01) :32-+
[9]   2 SPECIES OF DNA POLYMERASE ISOLATED FROM ESCHERICHIA COLI [J].
LEZIUS, AG ;
HENNIG, SB ;
MENZEL, C ;
METZ, E .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1967, 2 (01) :90-+
[10]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265
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