ENDOPLASMIC RETICULUM-GOLGI APPARATUS RELATIONSHIPS IN THE RAT SPERMATID

被引:30
作者
HERMO, L
CLERMONT, Y
RAMBOURG, A
机构
[1] Department of Anatomy, Mcgill University, Montreal, Quebec
来源
ANATOMICAL RECORD | 1979年 / 193卷 / 02期
关键词
D O I
10.1002/ar.1091930206
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
The appearance of the spermatid Golgi apparatus was studied, both in thin sections of rat testes fixed in glutaraldehyde and treated with either tannic acid or ferrocyanide‐reduced osmium, and in relatively thick sections (0.25‐0.5 μm) of glutaraldehyde fixed tissue impregnated with uranyl acetate followed by lead and copper citrate. With any one of these three procedures, the Golgi apparatus of young spermatids examined at the Golgi and cap phases appeared as a compact hemispherical mass whose base was located next to the developing acrosomic system. The cortical region of the hemisphere was composed mainly of several stacks of saccules. The central core or medullary region contained numerous vesicular and tubular membranous profiles. Numerous cisternae of the endoplasmic reticulum (ER) were closely applied to the surface of the Golgi apparatus. In thin sections, the ER cisternae were separated from the stacks of saccules by small spherical or elongated membranous profiles. In thick sections, most of these elements formed a network of tubules, some of which being continuous with ER cisternae. Cisternae of the endoplasmic reticulum near the cis‐face of the Golgi stacks also branched and traversed the cortical region of the Golgi apparatus through gaps seen between the stacks of saccules to reach the central core region. Some of these were closely applied to the trans elements of Golgi stacks although never in continuity with them. Finally, ER cisternae were found located within the stacks themselves between Golgi saccules; here the membranes of the closely apposed cisternae of the endoplasmic reticulum and Golgi saccules remained separated by a space of about 12 nm. Thus, in the spermatid, the endoplasmic reticulum was closely related with all components of the Golgi apparatus. Copyright © 1979 Wiley‐Liss, Inc.
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页码:243 / &
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