LABELED AGENTS FOR PET STUDIES OF THE DOPAMINERGIC SYSTEM - SOME QUALITY ASSURANCE METHODS, EXPERIENCE AND ISSUES

被引：12

作者：

PIKE, VW

KENSETT, MJ

TURTON, DR

WATERS, SL

SILVESTER, DJ

机构：

[1] MRC Cyclotron Unit, Hammersmith Hospital, London, W12 0HS, Ducane Road

来源：

APPLIED RADIATION AND ISOTOPES | 1990年 / 41卷 / 05期

关键词：

Labelling Agents - Pet Studies;

D O I：

10.1016/0883-2889(90)90009-6

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Practical methods are described for the quality assurance of three labelled agents (l-6-[18F]fluoro-DOPA, S-[N-methyl-11C]nomifensine and [O-methyl-11C]raclopride) now produced regularly for PET studies of the dopaminergic system in man. These include indirect methods for the initial determination of label position (e.g. 13C-NMR spectroscopy) and also direct methods for the assessment of chiral purity (TLC and HPLC) and the routine determination of radiochemical purity, chemical purity and specific activity (HPLC). Mass spectrometry has been used to identify some impurities. l-6-hydroxy-DOPA (a precursor in vivo of the neurotoxin, l-6-hydroxydopamine) has been detected by HPLC in some preparations of l-6-[18F]fluoro-DOPA. Formulated S-[N-methyl-11C]nomifensine has been found to decrease in radio-chemical purity with storage, whereas formulated [O-methyl-11C]raclopride has been found to be stable. Some quality assurance issues are discussed in relation to experience in the application of the described methods and the obtained results. © 1990.

引用

页码：483 / 492

页数：10

共 31 条

[1]

ADAM MJ, 1986, APPL RADIAT ISOTOPES, V37, P877

[2]

ADAM MJ, 1988, APPL RADIAT ISOTOPES, V39, P1203

[3]

ADAM MJ, 1986, J NUCL MED, V27, P1462

[4]

BRADY F, 1989, APPL RADIAT ISOTOPES, V40, P325

[5]

CHEN JJ, 1989, J NUCL MED, V30, P1249

[6] RADIOFLUORINATION WITH F-18-LABELED ACETYL HYPOFLUORITE - [F-18] L-6-FLUORODOPA [J].

CHIRAKAL, R ;

FIRNAU, G ;

COUSE, J ;

GARNETT, ES .

INTERNATIONAL JOURNAL OF APPLIED RADIATION AND ISOTOPES, 1984, 35 (07) :651-653

[7]

CHIUEH CC, 1988, P CHINESE ACAD MED S, V3, P48

[8] THE EFFECT OF RING-FLUORINATION ON THE RATE OF O-METHYLATION OF DIHYDROXYPHENYLALANINE (DOPA) BY CATECHOL-O-METHYLTRANSFERASE - SIGNIFICANCE IN THE DEVELOPMENT OF F-18-PETT SCANNING AGENTS [J].

CREVELING, CR ;

KIRK, KL .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 130 (03) :1123-1131

[9]

CROUZEL C, 1987, APPL RADIAT ISOTOPES, V38, P601

[10] KINETICS OF INVITRO DECARBOXYLATION AND THE INVIVO METABOLISM OF 2-F-18-FLUORODOPA AND 6-F-18-FLUORODOPA IN THE HOODED RAT [J].

CUMMING, P ;

HAUSSER, M ;

MARTIN, WRW ;

GRIERSON, J ;

ADAM, MJ ;

RUTH, TJ ;

MCGEER, EG .

BIOCHEMICAL PHARMACOLOGY, 1988, 37 (02) :247-250

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