PHAZE - A SHUTTLE VECTOR SYSTEM FOR THE DETECTION AND ANALYSIS OF IONIZING RADIATION-INDUCED MUTATIONS

被引：26

作者：

LUTZE, LH

WINEGAR, RA

机构：

[1] Laboratory of Radiobiology and Environmental Health, University of California, San Francisco

来源：

MUTATION RESEARCH | 1990年 / 245卷 / 04期

关键词：

Episome; human fibroblasts; Fibroblasts; episome; Ionizing radiation; PHaze; Shuttle vector;

D O I：

10.1016/0165-7992(90)90161-C

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have designed and constructed a shuttle vector system, pHAZE, that is maintained as an episome in normal human fibroblasts, has a low background mutation frequency, and is capable of detecting a spectrum of mutations, including deletions up to 8.3 kb. The efficacy of this system was demonstrated by using it to analyze mutations produced by X-rays, which induced both point mutations and large deletions in pHAZE. © 1990.

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页码：305 / 310

页数：6

相关论文

共 11 条

[1] ANALYSIS OF GENE-CONTROL SIGNALS BY DNA-FUSION AND CLONING IN ESCHERICHIA-COLI [J].

CASADABAN, MJ ;

COHEN, SN .

JOURNAL OF MOLECULAR BIOLOGY, 1980, 138 (02) :179-207

[2]

DEJONG PJ, 1988, P NATL ACAD SCI USA, V85, P3499

[3] RECOMBINANT SHUTTLE VECTORS FOR THE STUDY OF MUTATION IN MAMMALIAN-CELLS [J].

DUBRIDGE, RB ;

CALOS, MP .

MUTAGENESIS, 1988, 3 (01) :1-9

[4] ANALYSIS OF MUTATION IN HUMAN-CELLS BY USING AN EPSTEIN-BARR-VIRUS SHUTTLE SYSTEM [J].

DUBRIDGE, RB ;

TANG, P ;

HSIA, HC ;

LEONG, PM ;

MILLER, JH ;

CALOS, MP .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (01) :379-387

[5] BASE SUBSTITUTIONS, FRAMESHIFTS, AND SMALL DELETIONS CONSTITUTE IONIZING RADIATION-INDUCED POINT MUTATIONS IN MAMMALIAN-CELLS [J].

GROSOVSKY, AJ ;

DEBOER, JG ;

DEJONG, PJ ;

DROBETSKY, EA ;

GLICKMAN, BW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (01) :185-188

[6]

GROSOVSKY AJ, 1986, GENETICS, V113, P405

[7]

MILLER JH, 1978, OPERON, P31

[8] JOINING OF NONHOMOLOGOUS DNA DOUBLE STRAND BREAKS INVITRO [J].

PFEIFFER, P ;

VIELMETTER, W .

NUCLEIC ACIDS RESEARCH, 1988, 16 (03) :907-924

[9] MUTAGENESIS OF A SHUTTLE VECTOR PLASMID IN MAMMALIAN-CELLS [J].

RAZZAQUE, A ;

CHAKRABARTI, S ;

JOFFEE, S ;

SEIDMAN, M .

MOLECULAR AND CELLULAR BIOLOGY, 1984, 4 (03) :435-441

[10] A VECTOR THAT REPLICATES AS A PLASMID AND CAN BE EFFICIENTLY SELECTED IN LYMPHOBLASTS-B TRANSFORMED BY EPSTEIN-BARR VIRUS [J].

SUGDEN, B ;

MARSH, K ;

YATES, J .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (02) :410-413