PURIFICATION OF 2 THERMOSTABLE COMPONENTS OF MESSENGER-RIBONUCLEOPROTEIN PARTICLES (MESSENGER RNPS) FROM XENOPUS-LAEVIS OOCYTES, BELONGING TO A NOVEL CLASS OF RNA-BINDING PROTEINS

被引：32

作者：

DESCHAMPS, S

VIEL, A

DENIS, H

LEMAIRE, M

机构：

[1] Centre de Gènètique molèculaire, Laboratoire propre, Centre National de la Recherche Scientifique, F-91 198 Gif-sur-Yvette Cedex, associea l'Universite P. M. Curie (Paris VI)

来源：

FEBS LETTERS | 1991年 / 282卷 / 01期

关键词：

MESSENGERRNA-CONTAINING PARTICLE; MESSENGERRNA; AMINO ACID SEQUENCE; SEQUENCE HOMOLOGY; XENOPUS-LAEVIS; OOGENESIS;

D O I：

10.1016/0014-5793(91)80456-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have purified and partially sequenced two proteins from Xenopus laevis previtellogenic oocytes, belonging to messenger ribonucleoprotein particles (mRNPs). The purification procedure rests on the thermostability of these proteins, which remain soluble after heating the cell extracts at 80-degrees-C. The thermostable proteins can be identified with two of the most abundant components (mRNP3 and mRNP4) of the mRNPs, described by Darnbrough and Ford (1981) [Eur. J. Biochem. 118, 415-424]. mRNP3 and mRNP4 are homologous to each other, but to no other protein of known sequence. The abundance and semi-periodic distribution of proline residues in mRNP3 and mRNP4 sequences suggest that these RNA-binding proteins adopt an unusual type of conformation.

引用

页码：110 / 114

页数：5

共 23 条

[1] POLY(A), POLY(A) BINDING-PROTEIN AND THE REGULATION OF MESSENGER-RNA STABILITY
BERNSTEIN, P
ROSS, J
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (09) : 373 - 377
[2] NOVEL RNA INTERACTIONS WITH THE ELONGATION-FACTOR EF-TU - CONSEQUENCES FOR PROTEIN-SYNTHESIS AND TUF GENE-EXPRESSION
BOSCH, L
KRAAL, B
VANNOORT, JM
VANDELFT, J
TALENS, A
VIJGENBOOM, E
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 1985, 10 (08) : 313 - 316
[3] CLEVELAND DW, 1977, J BIOL CHEM, V252, P1102
[4] PROTEIN-KINASE ACTIVITY ASSOCIATED WITH STORED MESSENGER-RIBONUCLEOPROTEIN PARTICLES OF XENOPUS OOCYTES
CUMMINGS, A
SOMMERVILLE, J
[J]. JOURNAL OF CELL BIOLOGY, 1988, 107 (01) : 45 - 56
[5] IDENTIFICATION IN XENOPUS-LAEVIS OF A CLASS OF OOCYTE-SPECIFIC PROTEINS BOUND TO MESSENGER-RNA
DARNBROUGH, CH
FORD, PJ
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1981, 113 (03): : 415 - 424
[6] Davidson E. H., 1986, GENE ACTIVITY EARLY, V3rd
[7] Dayhoff M. O., 1968, ATLAS PROTEIN SEQUEN
[8] IDENTIFICATION OF A 60-KDA PHOSPHOPROTEIN THAT BINDS STORED MESSENGER-RNA OF XENOPUS OOCYTES
DEARSLEY, AL
JOHNSON, RM
BARRETT, P
SOMMERVILLE, J
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 150 (01): : 95 - 103
[9] BIOCHEMICAL-RESEARCH ON OOGENESIS - DISTRIBUTION OF TRANSFER RNA-LINKED PEPTIDES AND PROTEINS IN PREVITELLOGENIC OOCYTES OF XENOPUS-LAEVIS .22.
DENIS, H
LEMAIRE, M
[J]. BIOCHIMIE, 1987, 69 (05) : 495 - 509
[10] BIOCHEMICAL RESEARCH ON OOGENESIS .1. INTRACELLULAR DISTRIBUTION OF RNA IN SMALL OOCYTES OF XENOPUS-LAEVIS
DENIS, H
MAIRY, M
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1972, 25 (03): : 524 - +

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