THE SUBSTRUCTURE OF AGAROSE GELS AS PREPARED FOR ELECTROPHORESIS

被引：28

作者：

WHYTOCK, S

FINCH, J

机构：

[1] MRC Laboratory of Molecular Biology, Cambridge, CB2 2QH, Hills Road

来源：

BIOPOLYMERS | 1991年 / 31卷 / 09期

关键词：

D O I：

10.1002/bip.360310902

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gelled agarose was observed by freeze-fracture and electron microscopy. Aggregated double helices of agarose form a fibrous network that showed conformational variations over a range of agarose concentrations (0.3-2%). Diameters of fibers tend to increase with rising concentrations and the appearance of protuberances or cul-de-sacs in the network increases with falling concentration.

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页码：1025 / 1028

页数：4

相关论文

共 11 条

[1] AGAROSE DOUBLE HELIX AND ITS FUNCTION IN AGAROSE-GEL STRUCTURE [J].

ARNOTT, S ;

FULMER, A ;

SCOTT, WE ;

DEA, ICM ;

MOORHOUSE, R ;

REES, DA .

JOURNAL OF MOLECULAR BIOLOGY, 1974, 90 (02) :269-&

[2] SEPARATION OF LARGE DNA-MOLECULES BY CONTOUR-CLAMPED HOMOGENEOUS ELECTRIC-FIELDS [J].

CHU, G ;

VOLLRATH, D ;

DAVIS, RW .

SCIENCE, 1986, 234 (4783) :1582-1585

[3] AFFINITY INTERACTIONS BETWEEN AGAROSE AND BETA-1,4-GLYCANS - A MODEL FOR POLYSACCHARIDE ASSOCIATIONS IN ALGAL CELL-WALLS [J].

DEA, ICM ;

REES, DA .

CARBOHYDRATE POLYMERS, 1987, 7 (03) :183-224

[4] THEORETICAL-STUDIES OF DNA DURING GEL-ELECTROPHORESIS [J].

DEUTSCH, JM .

SCIENCE, 1988, 240 (4854) :922-924

[5] THE SIEVING OF SPHERES DURING AGAROSE-GEL ELECTROPHORESIS - QUANTITATION AND MODELING [J].

GRIESS, GA ;

MORENO, ET ;

EASOM, RA ;

SERWER, P .

BIOPOLYMERS, 1989, 28 (08) :1475-1484

[6] SEPARATION OF YEAST CHROMOSOME-SIZED DNAS BY PULSED FIELD GRADIENT GEL-ELECTROPHORESIS [J].

SCHWARTZ, DC ;

CANTOR, CR .

CELL, 1984, 37 (01) :67-75

[7] CONFORMATIONAL DYNAMICS OF INDIVIDUAL DNA-MOLECULES DURING GEL-ELECTROPHORESIS [J].

SCHWARTZ, DC ;

KOVAL, M .

NATURE, 1989, 338 (6215) :520-522

[8] EXCLUSION OF SPHERES BY AGAROSE GELS DURING AGAROSE-GEL ELECTROPHORESIS - DEPENDENCE ON THE SPHERES RADIUS AND THE GELS CONCENTRATION [J].

SERWER, P ;

HAYES, SJ .

ANALYTICAL BIOCHEMISTRY, 1986, 158 (01) :72-78

[9] ATYPICAL SIEVING OF OPEN CIRCULAR DNA DURING PULSED FIELD AGAROSE-GEL ELECTROPHORESIS [J].

SERWER, P ;

HAYES, SJ .

BIOCHEMISTRY, 1989, 28 (14) :5827-5832

[10] ANALYSIS OF CONVEX FERGUSON PLOTS IN AGAROSE-GEL ELECTROPHORESIS BY EMPIRICAL COMPUTER MODELING [J].

TIETZ, D ;

CHRAMBACH, A .

ELECTROPHORESIS, 1986, 7 (06) :241-250